大鼠骨骼肌单羧酸转运蛋白1（MCT1）的cDNA克隆
cDNA cloning of MCT1, a monocarboxylate transporter from rat skeletal muscle.
作者信息
Jackson V N, Price N T, Halestrap A P
机构信息
Department of Biochemistry, University of Bristol, UK.
出版信息
Biochim Biophys Acta. 1995 Sep 13;1238(2):193-6. doi: 10.1016/0005-2736(95)00160-5.
PMID:7548134
Abstract
PCR was used to amplify the coding region of CHO MCT1 cDNA. This was then used to screen a rat skeletal muscle cDNA library which lead to the isolation of a full length cDNA encoding MCT1 from rat. The cDNA derived amino acid sequence shows 94% and 86% identity to CHO and human MCT1, respectively.
摘要
采用聚合酶链反应（PCR）扩增中国仓鼠卵巢细胞（CHO）单羧酸转运蛋白1（MCT1）的编码区。然后用其筛选大鼠骨骼肌cDNA文库，从而分离出大鼠MCT1的全长cDNA。该cDNA推导的氨基酸序列与CHO及人MCT1的一致性分别为94%和86%。
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