Mizutani T, Hayashi M, Maeda A, Ishida K, Watanabe T, Namioka S
Department of Laboratory, Animal Science, Faculty of Veterinary Medicine, Hokkaido University, Sapporo.
Jpn J Vet Res. 1994 Aug;42(2):95-102.
The multiplication of mouse hepatitis virus (MHV) was inhibited by the treatment of infected cells with MgSO4 at concentrations higher than 50 mM. The inhibition of viral multiplication was more effective with the treatment of cells at the early stage of infection using MgSO4 than at the late stage. Viral adsorption to the cells was not inhibited by MgSO4 and pretreatment of the cells with MgSO4 did not show an inhibitory effect on the RNA synthesis of MHV. The synthesis of viral RNA was inhibited more effectively by the treatment of infected cells with MgSO4 at 0-2 and 2-4 h postinfection (p.i.) than at 4-6 h p.i. The present study suggests that the stage at which viral multiplication is susceptible to MgSO4 may be the early stage of viral transcription and that Mg2+ may be a useful tool for the analysis of the early stage of MHV infection.
用浓度高于50 mM的MgSO4处理感染细胞可抑制小鼠肝炎病毒（MHV）的增殖。在感染早期用MgSO4处理细胞比在晚期处理对病毒增殖的抑制更有效。MgSO4不抑制病毒对细胞的吸附，用MgSO4预处理细胞对MHV的RNA合成也没有抑制作用。在感染后0 - 2小时和2 - 4小时用MgSO4处理感染细胞比在感染后4 - 6小时处理更有效地抑制病毒RNA的合成。本研究表明，病毒增殖对MgSO4敏感的阶段可能是病毒转录的早期阶段，并且Mg2 +可能是分析MHV感染早期阶段的有用工具。
