Horiuchi M, Yamazaki N, Furuoka H, Matsui T, Nakagawa M, Ishiguro N, Shinagawa M
Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
J Vet Med Sci. 1995 Jun;57(3):577-80. doi: 10.1292/jvms.57.577.
Meningo-encephalitis in feedlot cattle sporadically occurred in the Tokachi area in northern Japan. The calves had been vaccinated intranasally with a mixed live-vaccine (infectious bovine rhinotracheitis virus, bovine viral diarrhea-mucosal disease virus, and parainfluenza 3 virus) for which intramuscular inoculation was indicated. Two additional live vaccines, bovine adenovirus type 7 and bovine respiratory syncytical virus, had been inoculated simultaneously. Eleven isolates of bovine herpesvirus type 1 were plaque-purified from two brains with fatal encephalitis; their viral DNAs were examined by restriction endonuclease analysis (REA) using PstI and HindIII. The REA patterns of the virus clones were almost identical to those of the vaccine strains 758-43, suggesting that the isolates from this outbreak of fatal encephalitis originated in the abnormally administered vaccine.
日本北部十胜地区的育肥牛偶尔会发生脑膜脑炎。这些小牛已通过鼻内接种混合活疫苗（传染性牛鼻气管炎病毒、牛病毒性腹泻-黏膜病病毒和副流感3病毒）进行了疫苗接种，而该疫苗的接种方式应为肌肉注射。同时还接种了另外两种活疫苗，即7型牛腺病毒和牛呼吸道合胞病毒。从两例患有致命性脑炎的牛脑中通过噬菌斑纯化获得了11株牛疱疹病毒1型毒株；使用PstI和HindIII通过限制性内切酶分析（REA）对其病毒DNA进行了检测。病毒克隆的REA模式与疫苗株758-43的模式几乎相同，这表明此次致命性脑炎疫情中的分离株源自接种方式不当的疫苗。
