al-Harbi M M, Qureshi S, Raza M, Ahmed M M, Giangreco A B, Shah A H
Department of Pharmacology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Eur J Cancer Prev. 1995 Aug;4(4):307-18. doi: 10.1097/00008469-199508000-00006.
The anticarcinogenic potential of anethole was studied in Ehrlich ascites tumour (EAT) in the paw of Swiss albino mice. The antitumour activity was evaluated from the cytotoxicity of EAT-cells in the paw and their biochemical changes were determined from nucleic acids, protein, malondialdehyde (MDA) and glutathione (NP-SH) concentrations. Furthermore, the observations on survival rate, tumour weight, its volume and body weight of EAT-bearing mice were made. The EAT-bearing paws were also evaluated for histopathological changes. Additional studies were undertaken on the cytological effects of anethole in order to establish its clastogenic and mitodepressive activity in normal mice. The results obtained in the present study revealed anethole to increase the survival time, reduce the tumour weight and volume and body weight of the EAT-bearing mice. It caused a significant cytotoxic effect in EAT cells in the paw, reduced the levels of nucleic acids and MDA, and increased NP-SH concentrations. The histopathological changes observed after treatment with anethole were comparable to the standard cytotoxic drug cyclophosphamide. The results on the frequency of micronuclei and the ratio of polychromatic erythrocytes to normochromatic erythrocytes showed anethole to be mitodepressive and non-clastogenic in the femoral cells of mice. Our results indicate the anticarcinogenic, cytotoxic and non=clastogenic nature of anethole. Further studies are warranted to explore the mode of action and safety of anethole for its possible use in cancer therapy.
在瑞士白化小鼠爪部的艾氏腹水瘤（EAT）模型中研究了茴香脑的抗癌潜力。通过爪部EAT细胞的细胞毒性评估其抗肿瘤活性，并从核酸、蛋白质、丙二醛（MDA）和谷胱甘肽（NP-SH）浓度测定其生化变化。此外，还观察了荷EAT小鼠的存活率、肿瘤重量、体积和体重。对荷EAT的爪部进行了组织病理学变化评估。为确定茴香脑对正常小鼠的致染色体断裂和有丝分裂抑制活性，还对其细胞学效应进行了额外研究。本研究获得的结果显示，茴香脑可延长荷EAT小鼠的存活时间，减轻肿瘤重量、体积和体重。它对爪部的EAT细胞产生显著的细胞毒性作用，降低核酸和MDA水平，并增加NP-SH浓度。茴香脑治疗后观察到的组织病理学变化与标准细胞毒性药物环磷酰胺相当。微核频率以及多染性红细胞与正染性红细胞的比例结果显示，茴香脑对小鼠股骨细胞具有有丝分裂抑制作用且无致染色体断裂作用。我们的结果表明茴香脑具有抗癌、细胞毒性和无致染色体断裂的特性。有必要进一步研究茴香脑的作用方式和安全性，以探讨其在癌症治疗中的潜在应用。
