通过体外肽刺激在麻疹血清阳性个体中证明病毒特异性CD8 +记忆T细胞。
Demonstration of virus-specific CD8+ memory T cells in measles-seropositive individuals by in vitro peptide stimulation.
作者信息
Nanan R, Carstens C, Kreth H W
机构信息
Universitäts-Kinderklinik, Würzburg, Germany.
出版信息
Clin Exp Immunol. 1995 Oct;102(1):40-5. doi: 10.1111/j.1365-2249.1995.tb06633.x.
Abstract
CD8+ cytotoxic T lymphocytes (CTL) in measles virus infection have been difficult to investigate due to the strong immunosuppressive effects exhibited by infectious measles virus in vitro. In order to circumvent immunosuppression we used predicted peptide epitopes to induce measles virus-specific CTL. This was done by screening the structural proteins of measles virus for HLA-A2.1 peptide-binding motifs with valine in position 2 and leucine in position 9. Synthetic peptides np210-218, np226-234, and np340-348 from the nucleoprotein, peptide hp29-37 from the haemagglutinin protein, and peptide pp 519-527 from the polymerase protein were synthesized and used to expand measles virus-specific CD8+ CTL in vitro. Induction of CTL with synthetic peptides was restricted to HLA-A2-positive peripheral blood mononuclear cells (PBMC) from measles-seropositive individuals. We conclude that this method is a useful tool to demonstrate memory CD8+ CTL in measles-seropositive adults and to evaluate the role of structural proteins in CTL responses against measles.
摘要
由于传染性麻疹病毒在体外表现出强烈的免疫抑制作用，因此很难对麻疹病毒感染中的CD8 + 细胞毒性T淋巴细胞（CTL）进行研究。为了规避免疫抑制，我们使用预测的肽表位来诱导麻疹病毒特异性CTL。通过筛选麻疹病毒的结构蛋白中第2位为缬氨酸、第9位为亮氨酸的HLA - A2.1肽结合基序来实现这一点。合成了来自核蛋白的合成肽np210 - 218、np226 - 234和np340 - 348，来自血凝素蛋白的肽hp29 - 37，以及来自聚合酶蛋白的肽pp 519 - 527，并用于在体外扩增麻疹病毒特异性CD8 + CTL。用合成肽诱导CTL仅限于来自麻疹血清阳性个体的HLA - A2阳性外周血单核细胞（PBMC）。我们得出结论，该方法是证明麻疹血清阳性成年人中记忆性CD8 + CTL以及评估结构蛋白在针对麻疹的CTL反应中的作用的有用工具。
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