Fleischer B, Kreth H W
Hum Immunol. 1983 Aug;7(4):239-48. doi: 10.1016/0198-8859(83)90061-7.
T cells were directly cloned from the cerebrospinal fluid (CSF) of a patient with acute measles encephalitis by limiting dilution in the presence of irradiated feeder cells and T cell growth factor (TCGF). A total of 42 colonies was established. Functional analysis revealed 27 of them to be derived from a cytotoxic T lymphocyte as demonstrated by the ability to exert phytohaemagglutinin (PHA)-dependent cytotoxicity against uninfected allogeneic PHA blasts. Twenty-three of the cytotoxic colonies were specific for measles virus and restricted to self HLA-A or -B antigens. Three clones were also found to give measles virus-specific proliferative responses. The results show that the CSF in measles encephalitis contains a highly enriched population of in vivo sensitized antigen-specific T cells. We propose that the clinical symptoms in measles encephalitis are caused by a T cell-mediated reaction against virus-infected brain cells.
通过在经辐照的饲养细胞和T细胞生长因子（TCGF）存在的情况下进行有限稀释，直接从一名急性麻疹脑炎患者的脑脊液（CSF）中克隆T细胞。共建立了42个集落。功能分析显示，其中27个集落来源于细胞毒性T淋巴细胞，这可通过对未感染的同种异体PHA母细胞发挥依赖于植物血凝素（PHA）的细胞毒性的能力来证明。23个细胞毒性集落对麻疹病毒具有特异性，并受自身HLA - A或 - B抗原限制。还发现3个克隆可产生麻疹病毒特异性增殖反应。结果表明，麻疹脑炎患者的脑脊液中含有高度富集的体内致敏抗原特异性T细胞群体。我们认为，麻疹脑炎的临床症状是由针对病毒感染脑细胞的T细胞介导反应引起的。
