Talbert P B, Adler H T, Parks D W, Comai L
University of Washington, Department of Botany, Seattle 98195-5325, USA.
Development. 1995 Sep;121(9):2723-35. doi: 10.1242/dev.121.9.2723.
The form of seed plants is determined by the growth of a number of meristems including apical meristems, leaf meristems and cambium layers. We investigated five recessive mutant alleles of a gene REVOLUTA that is required to promote the growth of apical meristems and to limit cell division in leaves and stems of Arabidopsis thaliana. REVOLUTA maps to the bottom of the fifth chromosome. Apical meristems of both paraclades (axillary shoots) and flowers of revoluta mutants frequently fail to complete normal development and form incomplete or abortive structures. The primary shoot apical meristem sometimes also arrests development early. Leaves, stems and floral organs, in contrast, grow abnormally large. We show that in the leaf epidermis this extra growth is due to extra cell divisions in the leaf basal meristem. The extent of leaf growth is negatively correlated with the development of a paraclade in the leaf axil. The thickened stems contain extra cell layers, arranged in rings, indicating that they may result from a cambium-like meristem. These results suggest that the REVOLUTA gene has a role in regulating the relative growth of apical and non-apical meristems in Arabidopsis.
种子植物的形态由包括顶端分生组织、叶分生组织和形成层在内的多个分生组织的生长所决定。我们研究了拟南芥中一个名为REVOLUTA的基因的五个隐性突变等位基因，该基因对于促进顶端分生组织的生长以及限制叶片和茎中的细胞分裂是必需的。REVOLUTA基因位于第五条染色体的末端。revoluta突变体的副枝（腋芽）和花的顶端分生组织常常无法完成正常发育，形成不完整或夭折的结构。主茎顶端分生组织有时也会过早停止发育。相比之下，叶片、茎和花器官则生长得异常大。我们发现，在叶片表皮中，这种额外的生长是由于叶片基部分生组织中额外的细胞分裂所致。叶片生长的程度与叶腋中副枝的发育呈负相关。加厚的茎含有额外的细胞层，呈环状排列，这表明它们可能源自类似形成层的分生组织。这些结果表明，REVOLUTA基因在调节拟南芥中顶端分生组织和非顶端分生组织的相对生长方面发挥作用。
