Rocchi E, Stella A M, Cassanelli M, Borghi A, Nardella N, Seium Y, Casalgrandi G
Department of Biological Chemistry, University of Buenos Aires, Argentina.
Eur J Clin Invest. 1995 Jul;25(7):510-4. doi: 10.1111/j.1365-2362.1995.tb01737.x.
The authors consider two groups of patients with overt sporadic porphyria cutanea tarda (PCT) from different continents, with the aim of evaluating the possible impairment of the liposoluble antioxidative system, given the possible synergic effect of porphyrins and iron in promoting oxidative cellular damage. Twenty-three Italian outpatients with overt sporadic PCT and 11 outpatients with PCT from Buenos Aires (Argentina) were matched with 60 patients with liver cirrhosis and 52 healthy Italian controls. Serum levels of alpha- and beta-carotene, cryptoxanthin, zeaxanthin, lutein, lycopene, retinol and alpha-tocopherol were detected by a high-performance liquid chromatographic technique devised in our laboratory, which afforded an accurate and simultaneous resolution of all these compounds. The results point to a significant reduction in plasma levels of alpha- and beta-carotene in both the PCT populations with respect not only to controls, but also to the cirrhotic population, which had more severe liver damage. Moreover, other carotenoids with proven antioxidative properties, like cryptoxanthin and lycopene, are greatly reduced in our PCT populations. This confirms the suggested synergic effect of iron and porphyrins in the oxidative intracellular damage with consequent depletion of antioxidative liposoluble molecules.
作者研究了来自不同大陆的两组显性散发性迟发性皮肤卟啉病（PCT）患者，旨在评估脂溶性抗氧化系统可能受到的损害，因为卟啉和铁在促进细胞氧化损伤方面可能存在协同作用。23名患有显性散发性PCT的意大利门诊患者和11名来自布宜诺斯艾利斯（阿根廷）的PCT门诊患者与60名肝硬化患者及52名健康的意大利对照者进行了匹配。通过我们实验室设计的高效液相色谱技术检测血清中α-和β-胡萝卜素、隐黄质、玉米黄质、叶黄素、番茄红素、视黄醇和α-生育酚的水平，该技术能够准确同时分离所有这些化合物。结果表明，与对照组相比，两个PCT患者群体的血浆α-和β-胡萝卜素水平均显著降低，与肝损伤更严重的肝硬化患者群体相比也是如此。此外，我们的PCT患者群体中其他具有抗氧化特性的类胡萝卜素，如隐黄质和番茄红素，也大幅减少。这证实了铁和卟啉在细胞内氧化损伤中存在协同作用，从而导致抗氧化脂溶性分子的消耗。
