植物核基因敲除揭示了细菌细胞分裂蛋白FtsZ（一种原始微管蛋白）的同源物在质体分裂中的作用。
Plant nuclear gene knockout reveals a role in plastid division for the homolog of the bacterial cell division protein FtsZ, an ancestral tubulin.
作者信息
Strepp R, Scholz S, Kruse S, Speth V, Reski R
机构信息
Institut Biologie II, Albert-Ludwigs-Universität Freiburg, Schänzlestrasse 1, D-79104 Freiburg, Germany.
出版信息
Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4368-73. doi: 10.1073/pnas.95.8.4368.
Abstract
Little is known about the division of eukaryotic cell organelles and up to now neither in animals nor in plants has a gene product been shown to mediate this process. A cDNA encoding a homolog of the bacterial cell division protein FtsZ, an ancestral tubulin, was isolated from the eukaryote Physcomitrella patens and used to disrupt efficiently the genomic locus in this terrestrial seedless plant. Seven out of 51 transgenics obtained were knockout plants generated by homologous recombination; they were specifically impeded in plastid division with no detectable effect on mitochondrial division or plant morphology. Implications on the theory of endosymbiosis and on the use of reverse genetics in plants are discussed.
摘要
关于真核细胞器的分裂，人们了解甚少。到目前为止，无论是在动物还是植物中，都尚未发现有基因产物介导这一过程。从真核生物小立碗藓中分离出一个编码细菌细胞分裂蛋白FtsZ（一种原始微管蛋白）同源物的cDNA，并用于有效破坏这种陆生无籽植物的基因组位点。在获得的51个转基因植株中，有7个是通过同源重组产生的基因敲除植株；它们在质体分裂方面受到特异性阻碍，而对线粒体分裂或植物形态没有可检测到的影响。文中还讨论了这一研究对共生起源理论以及植物反向遗传学应用的意义。
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