Whitfield L S, Hawkins T L, Goodfellow P N, Sulston J
Department of Genetics, University of Cambridge, United Kingdom.
Genomics. 1995 May 20;27(2):306-11. doi: 10.1006/geno.1995.1047.
Determination of 41.2 kb of Y chromosome genomic sequence has been made from a cosmid that spans the Yp pseudoautosomal boundary and includes 18.5 kb of sequence from the patient-defined sex-determining region of the Y chromosome. An AceDB database of the sequence and the analysis data have been produced as a resource for studies of the evolution and population genetics of the Y chromosome. Comparison of the 18.5 kb from the sex determining region to the sex determining region of mouse does not locate any areas of similarity outside SRY/Sry. Indeed, no coding regions other than those previously reported can be detected anywhere in the 41 kb. The Y-specific and pseudoautosomal portions of this sequence have different repeat sequence and GC contents: this may have relevance both to the events defining the pseudoautosomal boundary and to the course of sequence evolution in the absence of recombination.
已从一个跨越Yp假常染色体边界的黏粒中确定了41.2 kb的Y染色体基因组序列，该序列包括来自患者定义的Y染色体性别决定区域的18.5 kb序列。已建立该序列和分析数据的AceDB数据库，作为研究Y染色体进化和群体遗传学的资源。将来自性别决定区域的18.5 kb序列与小鼠的性别决定区域进行比较，未发现SRY/Sry以外的任何相似区域。实际上，在41 kb的任何位置都未检测到除先前报道的编码区域以外的其他编码区域。该序列的Y特异性和假常染色体部分具有不同的重复序列和GC含量：这可能与定义假常染色体边界的事件以及无重组情况下的序列进化过程都有关。
