用单克隆抗体进行血清类粘蛋白表型分析：台湾原住民群体中ORM1*Q0的多态性出现情况

Orosomucoid phenotyping with monoclonal antibodies:polymorphic occurrence of ORM1*Q0 in aboriginal Taiwanese populations.

作者信息

Umetsu K, Yuasa I, Harada A, Suzuki T, Pan I H, Ishida T, Saitou N, Horai S

机构信息

Department of Forensic Medicine, Yamagata University School of Medicine, Japan.

出版信息

Hum Hered. 1995 Jul-Aug;45(4):181-5. doi: 10.1159/000154286.

DOI:10.1159/000154286

PMID:7558048

Abstract

Three monoclonal antibodies (OR35, OR40 and OR48) against orosomucoid (ORM) were prepared for the phenotyping of the human ORM system. The OR35 and OR48 antibodies recognized ORM1 and ORM2 products, respectively. OR40 reacted strongly to the products of ORM1 but poorly to those of ORM2. With the help of these monoclonal antibodies, ORM phenotyping was performed on 658 individuals from nine subpopulations of aboriginal Taiwanese, with close attention to two individuals with an ORM1 Q0 homozygous phenotype. The ORM1*Q0 allele was found to be at a polymorphic frequency in eight of the nine subpopulations.

摘要

制备了三种抗血清类黏蛋白（ORM）的单克隆抗体（OR35、OR40和OR48）用于人类ORM系统的表型分析。OR35和OR48抗体分别识别ORM1和ORM2产物。OR40与ORM1的产物反应强烈，但与ORM2的产物反应较弱。借助这些单克隆抗体，对来自台湾原住民九个亚群的658名个体进行了ORM表型分析，特别关注了两名具有ORM1 Q0纯合表型的个体。发现ORM1*Q0等位基因在九个亚群中的八个中具有多态性频率。