Komiya A, Suzuki H, Aida S, Yatani R, Shimazaki J
Department of Urology, School of Medicine, Chiba University.
Jpn J Cancer Res. 1995 Jul;86(7):622-5. doi: 10.1111/j.1349-7006.1995.tb02443.x.
To study mutation of the CDKN2 gene in prostate cancer, samples from 51 Japanese patients and four human prostate cancer cell lines were examined by single-strand conformation polymorphism analysis and direct sequencing. Only one out of 51 (2%) patients revealed a mutation, which was a 24 bp deletion from the 5'-untranslated region to codon 3, resulting in loss of the initiation site. One of the four cell lines revealed a missense mutation, a GAC-->TAC (Asp-->Tyr) at codon 84. These results indicate that mutation of the CDKN2 gene is rare in prostate cancer and thus does not contribute significantly to the pathogenesis of human prostate cancer. Prostate cancer cell lines may acquire more frequent abnormality of the CDKN2 gene than tumor tissues.
为研究前列腺癌中CDKN2基因的突变情况，采用单链构象多态性分析和直接测序法对51例日本患者的样本及4种人前列腺癌细胞系进行了检测。51例患者中仅1例（2%）出现突变，该突变是从5'-非翻译区至密码子3处有24 bp的缺失，导致起始位点丢失。4种细胞系中有1种出现错义突变，即密码子84处的GAC→TAC（天冬氨酸→酪氨酸）。这些结果表明，CDKN2基因的突变在前列腺癌中较为罕见，因此对人类前列腺癌的发病机制贡献不大。前列腺癌细胞系可能比肿瘤组织更频繁地出现CDKN2基因异常。
