Saegusa M, Takano Y, Wakabayashi T, Okayasu I
Department of Pathology, School of Medicine, Kitasato University.
Jpn J Cancer Res. 1995 Aug;86(8):743-8. doi: 10.1111/j.1349-7006.1995.tb02463.x.
The significance of apoptosis in human gastric carcinomas was investigated in comparison with proliferative activity and p53 accumulation, using an in situ DNA nick end labeling method and immunohistochemistry for both Ki-67 antigen and p53 protein. Apoptotic labeling indices (LI) of 51 differentiated carcinomas (21 of early and 22 of advanced stage) were significantly lower than for 33 undifferentiated tumors (9 of early and 24 of advanced stage) (P < 0.05). In both types, apoptotic LI of advanced stage lesions were significantly higher than for the early stage cases (P < 0.005, P < 0.03). The distribution of apoptotic cells was different from that of Ki-67-positive cells, generally exhibiting an inverse correlation for areas of predominance. In contrast, there was no significant correlation between p53 immunoreactivity and either apoptotic LI or Ki-67 LI. It is concluded that in human gastric carcinomas the susceptibility to apoptosis is related to tumor cell differentiation and depth of invasion, and may play a role in selection of clonal subpopulations with high growth potential.
采用原位DNA缺口末端标记法以及针对Ki-67抗原和p53蛋白的免疫组织化学方法，通过与增殖活性和p53积聚情况相比较，研究细胞凋亡在人类胃癌中的意义。51例分化型癌（早期21例，晚期22例）的凋亡标记指数（LI）显著低于33例未分化肿瘤（早期9例，晚期24例）（P<0.05）。在这两种类型中，晚期病变的凋亡LI均显著高于早期病例（P<0.005，P<0.03）。凋亡细胞的分布与Ki-67阳性细胞不同，在优势区域通常呈负相关。相比之下，p53免疫反应性与凋亡LI或Ki-67 LI之间均无显著相关性。结论是，在人类胃癌中，细胞凋亡的易感性与肿瘤细胞分化和浸润深度有关，可能在具有高生长潜能的克隆亚群的选择中起作用。
