金黄色葡萄球菌8325/4主要自溶素的分子特征及功能分析
Molecular characterization and functional analysis of the major autolysin of Staphylococcus aureus 8325/4.
作者信息
Foster S J
机构信息
Department of Molecular Biology and Biotechnology, University of Sheffield, United Kingdom.
出版信息
J Bacteriol. 1995 Oct;177(19):5723-5. doi: 10.1128/jb.177.19.5723-5725.1995.
Abstract
The gene encoding the major autolysin of Staphylococcus aureus 8325/4 has been cloned, sequenced, and insertionally inactivated. The three-domain, 137,384-Da protein has a C-terminal glucosaminidase active site and is involved in cell separation, generalized cell lysis, and release of wall material at the cell surface. Expression occurs throughout growth and is stimulated by low temperatures and in the presence of 1 M NaCl.
摘要
编码金黄色葡萄球菌8325/4主要自溶素的基因已被克隆、测序并插入失活。这种由三个结构域组成、分子量为137,384道尔顿的蛋白质具有一个C端氨基葡萄糖苷酶活性位点，参与细胞分离、普遍的细胞裂解以及细胞壁物质在细胞表面的释放。该基因在整个生长过程中都有表达，并且在低温和1M氯化钠存在的情况下会受到刺激。
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Mol Microbiol. 1993 Apr;8(2):299-310. doi: 10.1111/j.1365-2958.1993.tb01574.x.
2
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J Bacteriol. 1993 Mar;175(5):1493-9. doi: 10.1128/jb.175.5.1493-1499.1993.
3
Effect of physiological conditions on the autolysis of Staphylococcus aureus strains.生理条件对金黄色葡萄球菌菌株自溶的影响。
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4
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Infect Immun. 1994 Apr;62(4):1406-9. doi: 10.1128/iai.62.4.1406-1409.1994.
5
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6
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Proc Natl Acad Sci U S A. 1995 Jan 3;92(1):285-9. doi: 10.1073/pnas.92.1.285.
7
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J Bacteriol. 1982 Aug;151(2):636-47. doi: 10.1128/jb.151.2.636-647.1982.
8
Mechanism of autolysis of isolated cell walls of Staphylococcus aureus.金黄色葡萄球菌分离细胞壁的自溶机制。
J Bacteriol. 1969 Feb;97(2):837-47. doi: 10.1128/jb.97.2.837-847.1969.
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10
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