Tsai S T, Jin Y T, Wu T C
Department of Otolaryngology, National Cheng Kung University Medical College, Taipan, Taiwan.
J Virol Methods. 1995 Jul;54(1):67-74. doi: 10.1016/0166-0934(95)00030-x.
A PCR-derived digoxigenin-labeled DNA probe was used for for Epstein-Barr early RNA (EBER) in situ hybridization in formalin-fixed paraffin-embedded tissues. The results showed that the hybridization signal was morphologically distinct and the intensity of signal was comparable with those by RNA riboprobe. The advantages of using PCR-derived DNA probes for EBER in situ hybridization include: (1) the synthesis of digoxigenin-labeled DNA probes is easy and simple by PCR; (2) the labeled amplification product can be used as a probe without further purification; (3) DNA probes are potentially more stable than RNA probes; and (4) the preparation of DNA probes is relatively efficient and rapid. It is concluded that this technique is an ideal candidate for detection of EBER expression in clinical specimens.
采用聚合酶链反应（PCR）衍生的地高辛标记DNA探针，对福尔马林固定石蜡包埋组织进行爱泼斯坦-巴尔早期RNA（EBER）原位杂交。结果显示，杂交信号在形态上有明显差异，信号强度与RNA核糖探针相当。使用PCR衍生的DNA探针进行EBER原位杂交的优点包括：（1）通过PCR合成地高辛标记的DNA探针简便易行；（2）标记的扩增产物无需进一步纯化即可用作探针；（3）DNA探针可能比RNA探针更稳定；（4）DNA探针的制备相对高效快速。得出结论，该技术是检测临床标本中EBER表达的理想方法。
