Sigler L, de la Maza L M, Tan G, Egger K N, Sherburne R K
University of Alberta Microfungus Collection and Herbarium, Devonian Botanic Garden, Edmonton, Canada.
J Clin Microbiol. 1995 Aug;33(8):1979-83. doi: 10.1128/jcm.33.8.1979-1983.1995.
The presence of clamp connections on hyphae and the development of fruiting bodies in culture are primary characters which allow identification of the basidiomycete Schizophyllum commune in cases of human infection. The diagnostic problems presented by a nonclamped, nonfruiting isolate from a dense mass in the right upper lobe of the lung in a female with a past history of pulmonary tuberculosis and diabetes are described. Several features of the isolated fungus, including rapid growth rate and white, dense, cottony colonies, tolerance to the fungicide benomyl at a concentration of 10 micrograms/ml, and susceptibility to cycloheximide at 400 micrograms/ml, suggested that it might be a basidiomycete. Transmission electron microscopy showed the presence of a dolipore septum with perforate pore cap characteristic of fungi in the class Holobasidiomycetes. However, species identification remained elusive until compatibility tests with known single-basidiospore isolates confirmed the identification of the sterile lung isolate as S. commune. Sequence analysis of the 5' internal transcribed spacer region of ribosomal DNA further supported conspecificity.
菌丝上存在锁状联合以及培养物中产生子实体是主要特征，可用于在人类感染病例中鉴定担子菌裂褶菌。描述了一名有肺结核和糖尿病病史的女性右肺上叶致密团块中分离出的无锁状联合、无子实体的菌株所带来的诊断问题。分离出的真菌的几个特征，包括生长速度快、白色致密棉絮状菌落、对浓度为10微克/毫升的杀菌剂苯菌灵有耐受性以及对400微克/毫升的放线菌酮敏感，表明它可能是一种担子菌。透射电子显微镜显示存在具有全担子菌纲真菌特征的多孔隔膜和穿孔孔帽。然而，在与已知的单担孢子分离株进行相容性试验确认无菌肺分离株为裂褶菌之前，物种鉴定一直难以确定。核糖体DNA的5' 内转录间隔区的序列分析进一步支持了同种性。
