Comstock L E, Maneval D, Panigrahi P, Joseph A, Levine M M, Kaper J B, Morris J G, Johnson J A
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore.
Infect Immun. 1995 Jan;63(1):317-23. doi: 10.1128/iai.63.1.317-323.1995.
Vibrio cholerae O139 Bengal, although closely related to V. cholerae O1 El Tor, produces a polysaccharide capsule and has a distinct O antigen. We have identified a chromosomal region of at least 11 kb, as defined by three TnphoA mutations, that is required for the expression of both polysaccharides. Electron microscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis show that these TnphoA mutants have lost the abilities both to express capsule and to produce lipopolysaccharide beyond the core oligosaccharide. Reactivity with O139 typing serum and resistance to serum are also lost in the mutants. DNA probes for this region do not hybridize with O1 V. cholerae but do react with other vibrios, implying that the region was recently acquired.
霍乱弧菌O139孟加拉型，尽管与霍乱弧菌O1埃尔托生物型密切相关，但能产生一种多糖荚膜且具有独特的O抗原。我们已经鉴定出一个至少11 kb的染色体区域，该区域由三个TnphoA突变所界定，是两种多糖表达所必需的。电子显微镜和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示，这些TnphoA突变体既丧失了表达荚膜的能力，也丧失了产生核心寡糖以外的脂多糖的能力。突变体与O139分型血清的反应性和对血清的抗性也丧失了。该区域的DNA探针与O1霍乱弧菌不杂交，但与其他弧菌有反应，这意味着该区域是最近获得的。
