Corcoran G D, Tovey D G, Moody A H, Chiodini P L
Department of Clinical Parasitology, Hospital for Tropical Diseases, London.
J Clin Pathol. 1995 Aug;48(8):725-7. doi: 10.1136/jcp.48.8.725.
To detect enteric microsporidia in faecal specimens from patients with the acquired immunodeficiency syndrome (AIDS), and to identify the spores to species level without using invasive procedures.
Formalised faecal preparations were examined using a modification of the strong trichrome staining method to demonstrate microsporidian spores. Six positive specimens were prepared for electron microscopy by emulsification and separation using a 9% Ficoll gradient.
The modified staining technique readily identified microsporidian spores. Spores of different species showed variation in size. Identification using electron microscopy was successful for five of the six positive specimens examined. It was unsuccessful for one specimen in which spores were less abundant on initial staining.
The modified strong trichrome staining method is a useful way of detecting spores of intestinal microsporidia in faecal specimens. Variation in spore size may permit provisional identification by light microscopy. Electron microscopic examination of faecal preparations is useful for identifying spores to species level.
检测获得性免疫缺陷综合征（AIDS）患者粪便标本中的肠道微孢子虫，并在不采用侵入性操作的情况下将孢子鉴定到种水平。
使用改良的强三色染色法检查固定后的粪便标本，以显示微孢子虫孢子。通过用9%菲可梯度液乳化和分离，对6份阳性标本进行电子显微镜检查准备。
改良染色技术能轻易鉴定出微孢子虫孢子。不同种的孢子大小有差异。在所检查的6份阳性标本中，有5份通过电子显微镜成功鉴定。有1份标本最初染色时孢子数量较少，鉴定未成功。
改良的强三色染色法是检测粪便标本中肠道微孢子虫孢子的一种有用方法。孢子大小的差异可能有助于通过光学显微镜进行初步鉴定。对粪便标本进行电子显微镜检查有助于将孢子鉴定到种水平。
