Cross D L, Smith G L
Department of Oral Medicine and Oral Pathology, University of Edinburgh, Infirmary Street, Scotland, UK.
J Clin Periodontol. 1995 Jul;22(7):569-77. doi: 10.1111/j.1600-051x.1995.tb00806.x.
The aims of this study were to compare the prevalence of suspected periodontal pathogens in subgingival plaque from 29 HIV seropositive and 27 control subjects and to determine the association of these bacteria with periodontal destruction. Subgingival plaque was collected from the mesiobuccal sites of all teeth, except 3rd molars. Bacteria were identified and enumerated using non-isotopic whole chromosomal DNA probes and a colony lift method. At baseline, HIV seropositive subjects had significantly higher mean % of Porphyromonas gingivalis than control subjects. This difference could be attributed to a subgroup of HIV seropositive subjects with widespread attachment loss. No correlations were observed between the mean %s of DNA probe species and mean attachment loss, CD4 and CD8 T lymphocyte counts or CD4: CD8 ratio. No significant microbiological differences were detected between active and control sites in HIV seropositive subjects on a longitudinal basis. There appeared to be an inverse relationship between the mean %s of P. gingivalis and V. parvula, with respect to progression of HIV infection. The ability of microbiological parameters to predict site-specific breakdown in HIV seropositive subjects requires further investigation.
本研究的目的是比较29名HIV血清阳性患者和27名对照受试者龈下菌斑中可疑牙周病原体的患病率，并确定这些细菌与牙周破坏之间的关联。除第三磨牙外，从所有牙齿的近中颊侧部位采集龈下菌斑。使用非同位素全染色体DNA探针和菌落转移法对细菌进行鉴定和计数。在基线时，HIV血清阳性受试者牙龈卟啉单胞菌的平均百分比显著高于对照受试者。这种差异可能归因于一组有广泛附着丧失的HIV血清阳性受试者。未观察到DNA探针菌种的平均百分比与平均附着丧失、CD4和CD8 T淋巴细胞计数或CD4:CD8比值之间存在相关性。在纵向研究中，未在HIV血清阳性受试者的活动部位和对照部位之间检测到显著的微生物学差异。就HIV感染的进展而言，牙龈卟啉单胞菌和小韦荣球菌的平均百分比之间似乎存在反比关系。微生物学参数预测HIV血清阳性受试者特定部位破坏的能力需要进一步研究。
