Schedle A, Samorapoompichit P, Rausch-Fan X H, Franz A, Füreder W, Sperr W R, Sperr W, Ellinger A, Slavicek R, Boltz-Nitulescu G, Valent P
Institute of Histology and Embryology, University of Vienna, Austria.
J Dent Res. 1995 Aug;74(8):1513-20. doi: 10.1177/00220345950740081301.
Recent data suggest that under certain conditions, various metal cations are released from dental alloys. These ions may produce adverse effects in various cell types in vivo. In this study, the cytopathogenic effects of 13 metal cations on murine L-929 fibroblasts, human gingival fibroblasts, and human tissue mast cells were analyzed in vitro. Several metal cations (dose range, from 0.0033 to 1.0 mmol/L) were found to induce dose-dependent inhibition of 3H-thymidine incorporation into cultured fibroblasts. The rank order of potency (lowest observed effect level, LOEL) for L-929 fibroblasts was: Ag+ > Pt4+ > Co2+ > In3+ > Ga3+ > Au3+ > Cu2+ > Ni2+ > Zn2+ > Pd2+ > Mo5+ > Sn2+ > Cr2+. A similar rank order of potency was obtained for primary human gingival fibroblasts: Pt4+ > Ag+ > Au3+ > In3+ > Ga3+ > Ni2+ > Co2+ > Zn2+ > Cu2+ > Cr2+ > Pd2+ > Mo5+ > Sn2+. In primary human mast cells, Ag+ and Au3+ caused dose-dependent toxic histamine release, whereas the other metal cations were ineffective over the dose range tested. To investigate the mechanism of metal cation-induced effects, we performed DNA as well as electron microscopic analyses on cultured fibroblasts. Both the DNA pattern and the ultrastructure of L-929 cells and gingival fibroblasts after exposure to cytopathogenic metal cations revealed signs of necrosis but no signs of apoptosis. Together, our data provide evidence that various metal cations produce dose-dependent cytopathogenic effects in distinct cell types, including human gingival fibroblasts and human tissue mast cells.(ABSTRACT TRUNCATED AT 250 WORDS)
近期数据表明，在某些条件下，牙科合金会释放出各种金属阳离子。这些离子可能在体内对多种细胞类型产生不良影响。在本研究中，体外分析了13种金属阳离子对小鼠L-929成纤维细胞、人牙龈成纤维细胞和人组织肥大细胞的细胞致病作用。发现几种金属阳离子（剂量范围为0.0033至1.0 mmol/L）可诱导对3H-胸腺嘧啶核苷掺入培养成纤维细胞的剂量依赖性抑制。L-929成纤维细胞的效力等级顺序（最低观察效应水平，LOEL）为：Ag+ > Pt4+ > Co2+ > In3+ > Ga3+ > Au3+ > Cu2+ > Ni2+ > Zn2+ > Pd2+ > Mo5+ > Sn2+ > Cr2+。原代人牙龈成纤维细胞也得到了类似的效力等级顺序：Pt4+ > Ag+ > Au3+ > In3+ > Ga3+ > Ni2+ > Co2+ > Zn2+ > Cu2+ > Cr2+ > Pd2+ > Mo5+ > Sn2+。在原代人肥大细胞中，Ag+和Au3+引起剂量依赖性毒性组胺释放，而其他金属阳离子在测试剂量范围内无效。为了研究金属阳离子诱导效应的机制，我们对培养的成纤维细胞进行了DNA以及电子显微镜分析。暴露于细胞致病金属阳离子后，L-929细胞和牙龈成纤维细胞的DNA模式和超微结构均显示出坏死迹象，但无凋亡迹象。总之，我们的数据提供了证据，表明各种金属阳离子在包括人牙龈成纤维细胞和人组织肥大细胞在内的不同细胞类型中产生剂量依赖性细胞致病作用。（摘要截断于250字）
