Yang H H, Aulerich R J, Helferich W, Yamini B, Chou K C, Miller E R, Bursian S J
Department of Animal Science, Michigan State University, East Lansing 48824, USA.
J Appl Toxicol. 1995 May-Jun;15(3):223-32. doi: 10.1002/jat.2550150314.
Tamoxifen (TAM), which binds to estrogen receptors and can act as an estrogen antagonist, was incorporated into the diets of swine and mink to determine if it would ameliorate the effects of the estrogenic mycotoxin zearalenone (ZEN). Sows and female mink were fed diets containing 2 ppm (swine) or 20 ppm (mink) ZEN and/or 10 ppm TAM from day 30 of gestation through weaning (swine) or from 2 months prior to breeding through weaning (mink). The diets containing ZEN and/or TAM did not adversely affect reproduction in the sows. Although some hyperestrogenic effects on testes, uterine and ovarian weights were observed in the F1 piglets at 21 days of age, subsequent breeding performance was not affected. All the female mink exposed to ZEN mated, but only 25% whelped. No mink fed TAM (singly or in combination with ZEN) mated. Necropsy of these unmated females fed TAM revealed consistent severe pyometra. Histological examination of the reproductive tracts of the ZEN, TAM and ZEN + TAM-treated mink showed similar alterations, including ovarian follicular atrophy and degeneration, and mild to severe uterine atrophy, pyometra and endometritis. The results of these studies indicate that TAM was not effective in ameliorating the hyperestrogenic effects of ZEN in swine and mink, but rather it acted as an estrogen agonist.
他莫昔芬（TAM）可与雌激素受体结合并作为雌激素拮抗剂，将其添加到猪和水貂的日粮中，以确定它是否能改善雌激素性霉菌毒素玉米赤霉烯酮（ZEN）的影响。从妊娠第30天到断奶（猪）或从配种前2个月到断奶（水貂），给母猪和雌性水貂饲喂含有2 ppm（猪）或20 ppm（水貂）ZEN和/或10 ppm TAM的日粮。含ZEN和/或TAM的日粮对母猪的繁殖没有不利影响。虽然在21日龄的F1仔猪中观察到对睾丸、子宫和卵巢重量有一些高雌激素效应，但随后的繁殖性能并未受到影响。所有接触ZEN的雌性水貂都交配了，但只有25%产仔。没有饲喂TAM（单独或与ZEN联合）的水貂交配。对这些未交配且饲喂TAM的雌性水貂进行尸检发现一致的严重子宫积脓。对ZEN、TAM和ZEN + TAM处理的水貂生殖道进行组织学检查显示出相似的变化，包括卵巢卵泡萎缩和退化，以及轻度至重度子宫萎缩、子宫积脓和子宫内膜炎。这些研究结果表明，TAM在改善猪和水貂中ZEN的高雌激素效应方面无效，相反，它起到了雌激素激动剂的作用。
