Kleiman de Pisarev D L, Ferramola de Sancovich A M, Sancovich H A
Departamentos de Quimica Biológica, Facultad de Medicina, Buenos Aires, Argentina.
J Endocrinol Invest. 1995 Apr;18(4):271-6. doi: 10.1007/BF03347812.
The functional thyroid status of hexachlorobenzene (HCB)-treated rats was studied. HCB caused a depletion of serum thyroxine (T4), but did not change L-3,5,3'-triiodothyronine (T3) levels in serum of rats. The activities of the thyroid regulated mitochondrial enzyme L-glycerolphosphate dehydrogenase (LGPD) and cytosolic enzymes, malic enzyme (ME), glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) were assayed in livers of normal and HCB (100 mg/100 g bw) treated Wistar rats. Mitochondrial LGPD activity did not change significantly, however ME, 6GPD and G6PD were induced by HCB only in non-thyroidectomized animals. The absence of cytosolic enzymes induction in thyroidectomized rats treated with HCB indicates that HCB is not intrinsically thyromimetic. The induction of hepatic ME, G6PD and 6PGD activities in HCB thyroidectomized rats was dependent on the presence of thyroid hormone. The unchanged activity of mitochondrial LGPD in contrast to the increased activities of the cytosolic enzymes ME, G6PD and 6PGD is not consistent with a shift in functional thyroid status following HCB treatment.
研究了六氯苯（HCB）处理大鼠的甲状腺功能状态。HCB导致大鼠血清甲状腺素（T4）减少，但对大鼠血清中L-3,5,3'-三碘甲状腺原氨酸（T3）水平无影响。在正常和经HCB（100mg/100g体重）处理的Wistar大鼠肝脏中，检测了甲状腺调节的线粒体酶L-甘油磷酸脱氢酶（LGPD）以及胞质酶苹果酸酶（ME）、葡萄糖-6-磷酸脱氢酶（G6PD）和6-磷酸葡萄糖酸脱氢酶（6PGD）的活性。线粒体LGPD活性无显著变化，然而ME、6GPD和G6PD仅在未切除甲状腺的动物中被HCB诱导。在经HCB处理的切除甲状腺的大鼠中未出现胞质酶诱导现象，这表明HCB本身并非甲状腺模拟物。在经HCB处理的切除甲状腺的大鼠中，肝脏ME、G6PD和6PGD活性的诱导依赖于甲状腺激素的存在。与胞质酶ME、G6PD和6PGD活性增加形成对比的是，线粒体LGPD活性未改变，这与HCB处理后甲状腺功能状态的改变不一致。
