Hesselton R M, Greiner D L, Mordes J P, Rajan T V, Sullivan J L, Shultz L D
Department of Pediatrics, University of Massachusetts Medical School, Worcester, USA.
J Infect Dis. 1995 Oct;172(4):974-82. doi: 10.1093/infdis/172.4.974.
Inbred C.B-17-scid/scid mice accept human peripheral blood mononuclear cell (PBMC) xenografts and are susceptible to human immunodeficiency virus type 1 (HIV-1) infection, but low levels of PBMC engraftment impede use of this system in HIV research. This report describes the effect of host strain background on human PBMC engraftment and HIV infectivity in scid mice. Back-crossing the scid mutation to the NOD/Lt strain (designated NOD/LtSz-scid/scid) increased the percentage of engrafted human PBMC in recipient spleens by 5- to 10-fold compared with that in C.B-17-scid/scid stock. Four weeks after human PBMC-injected mice were infected with HIV-1, 79% of NOD/LtSz-scid/scid spleens harbored replicating virus compared with only 39% of spleens in C.B-17-scid/scid mice. The NOD/LtSz-scid/scid mouse may provide a useful small animal model for studies of HIV-1.
近交系C.B-17-scid/scid小鼠能接受人外周血单个核细胞（PBMC）异种移植，且易感染1型人类免疫缺陷病毒（HIV-1），但PBMC低水平植入阻碍了该系统在HIV研究中的应用。本报告描述了宿主品系背景对scid小鼠中人PBMC植入及HIV感染性的影响。将scid突变回交至NOD/Lt品系（命名为NOD/LtSz-scid/scid），与C.B-17-scid/scid原种相比，受体脾脏中植入的人PBMC百分比增加了5至10倍。在注射人PBMC的小鼠感染HIV-1四周后，79%的NOD/LtSz-scid/scid小鼠脾脏中含有复制病毒，而C.B-17-scid/scid小鼠脾脏中这一比例仅为39%。NOD/LtSz-scid/scid小鼠可能为HIV-1研究提供一种有用的小动物模型。
