McBurney M I, Apps K V, Finegood D T
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada.
J Nutr. 1995 Oct;125(10):2571-6. doi: 10.1093/jn/125.10.2571.
Long-term ingestion of high fiber diets is associated with reduced glucose concentrations during fasting and improved glucose tolerance (KG) in humans. Our objective was to determine if the beneficial effects of fiber were attributable to increased production of short-chain fatty acid (SCFA) in the large intestine. Effects of SCFA on insulin sensitivity (SI), glucose effectiveness (SG), KG and baseline concentrations of glucose, insulin, glucagon and free fatty acids were examined in unfed 20-50 kg pigs (n = 6). Animals randomly received separate portal infusions (0.32 mL.min-1) of saline, acetic, propionic, and butyric acid solutions (0.01 mmol SCFA kg body weight-1.min-1) for 7-d periods. On d 7, somatostatin and tolbutamide modified frequently sampled intravenous glucose tolerance tests were performed. SI and SG were calculated using Bergman's Minimal-Model. KG was determined by regression of log glucose curve versus time. SI, SG and KG values did not differ among the treatments (P > 0.05). Baseline concentrations of glucose, insulin, glucagon and free fatty acids were unaffected by infusion treatment (P > 0.05). Our results suggest that SCFA delivery is not directly responsible for improvements in glucose metabolism observed with long-term ingestion of high fiber diets.
长期摄入高纤维饮食与人类空腹血糖浓度降低和糖耐量改善（KG）相关。我们的目的是确定纤维的有益作用是否归因于大肠中短链脂肪酸（SCFA）产生的增加。在未进食的20 - 50千克猪（n = 6）中，研究了SCFA对胰岛素敏感性（SI）、葡萄糖效能（SG）、KG以及葡萄糖、胰岛素、胰高血糖素和游离脂肪酸基线浓度的影响。动物随机接受为期7天的门静脉单独输注（0.32毫升·分钟⁻¹）生理盐水、乙酸、丙酸和丁酸溶液（0.01毫摩尔SCFA·千克体重⁻¹·分钟⁻¹）。在第7天，进行生长抑素和甲苯磺丁脲改良的频繁采样静脉葡萄糖耐量试验。使用伯格曼最小模型计算SI和SG。通过葡萄糖对数曲线与时间的回归确定KG。各处理间SI、SG和KG值无差异（P > 0.05）。输注处理对葡萄糖、胰岛素、胰高血糖素和游离脂肪酸的基线浓度无影响（P > 0.05）。我们的结果表明，SCFA的输送并非长期摄入高纤维饮食所观察到的葡萄糖代谢改善的直接原因。
