Fu Y, He F, Zhang S, Jiao X
Institute of Occupational Medicine, Chinese Academy of Preventive Medicine, Beijing.
Neurotoxicol Teratol. 1995 Jul-Aug;17(4):413-8. doi: 10.1016/0892-0362(94)00078-r.
Arthrinium fungi were cultivated from the samples of mildewed sugarcane which caused acute encephalopathy and delayed dystonia in children. The Arthrinium cultures (AC) contained 5 mg/ml 3-nitropropionic acid (3-NPA) after being inactivated and concentrated. A neuropathological study was carried out in rats intoxicated with 3-NPA and AC, respectively. Consistent bilateral striatal necrosis was found in rats with both poisonings, and the severity was well correlated with persistent recumbency which was a clinical indicator of the development of morphological brain lesions. A reproducible animal model of striatal damage has been produced in rats by IP injections of 10 mg/kg 3-NPA, 6 times a day, with an interval of 1.5 h for 3-5 days. The clinical and neuropathological manifestations in rats dosed with AC were nearly the same as those dosed with 3-NPA. The striatal lesions induced by 3-NPA and AC in poisoned rats were in accordance with the bilateral lenticular hypodensity found by CT scanning in patients of mildewed sugarcane poisoning with delayed dystonia. This neuropathological evidence supports previous epidemiological and mycological findings which indicate that 3-NPA is the possible pathogen of acute mildewed sugarcane poisoning.
从导致儿童急性脑病和迟发性肌张力障碍的霉变甘蔗样本中培养出节菱孢属真菌。节菱孢属培养物（AC）在灭活和浓缩后含有5毫克/毫升的3-硝基丙酸（3-NPA）。分别对用3-NPA和AC中毒的大鼠进行了神经病理学研究。两种中毒的大鼠均发现双侧纹状体一致的坏死，其严重程度与持续卧倒密切相关，而持续卧倒是脑形态学损伤发展的临床指标。通过腹腔注射10毫克/千克的3-NPA，每天6次，间隔1.5小时，持续3至5天，在大鼠中建立了一种可重复的纹状体损伤动物模型。给予AC的大鼠的临床和神经病理学表现与给予3-NPA的大鼠几乎相同。3-NPA和AC在中毒大鼠中诱导的纹状体病变与霉变甘蔗中毒伴迟发性肌张力障碍患者CT扫描发现的双侧豆状核低密度一致。这一神经病理学证据支持了先前的流行病学和真菌学研究结果，即3-NPA是急性霉变甘蔗中毒的可能病原体。
