Altomare D A, Guo K, Cheng J Q, Sonoda G, Walsh K, Testa J R
Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA.
Oncogene. 1995 Sep 21;11(6):1055-60.
We isolated mouse cDNA clones containing the entire coding region of the putative oncogene Akt2. Sequence analysis revealed that, like its human homolog, Akt2 encodes a protein-serine/threonine kinase containing a pleckstrin homology domain at its amino terminus. Fluorescence in situ hybridization of the mouse cDNA to rodent metaphase spreads demonstrated that the Akt2 gene maps to mouse chromosome band 7B1 and rat chromosome 1q22. Expression levels of mouse Akt2 mRNA and Akt2 protein varied among tissues, with the highest levels in skeletal muscle. Akt2 expression was low in a multipotent fibroblast cell line, but it was upregulated when these cells were transformed with Myod and induced to differentiate into myocytes. These data demonstrate that Akt2 expression is activated during cellular differentiation and suggest that it functions in the signaling pathways of some adult tissues.
我们分离出了包含假定癌基因Akt2完整编码区的小鼠cDNA克隆。序列分析显示，与其人类同源物一样，Akt2编码一种蛋白丝氨酸/苏氨酸激酶，其氨基末端含有一个普列克底物蛋白同源结构域。将小鼠cDNA与啮齿动物中期染色体铺展进行荧光原位杂交表明，Akt2基因定位于小鼠染色体7B1带和大鼠染色体1q22。小鼠Akt2 mRNA和Akt2蛋白的表达水平在不同组织中有所不同，在骨骼肌中水平最高。Akt2在多能成纤维细胞系中的表达较低，但当这些细胞用Myod转化并诱导分化为肌细胞时，其表达上调。这些数据表明Akt2表达在细胞分化过程中被激活，并提示它在某些成年组织的信号通路中发挥作用。
