McGregor A, Schio F, Beaton S, Boulton V, Perman M, Gilbert G
Department of Clinical Microbiology, Westmead Hospital, Sydney.
Pathology. 1995 Apr;27(2):172-6. doi: 10.1080/00313029500169822.
The MicroScan WalkAway is an automated bacterial identification and susceptibility testing system that has only recently been marketed in Australasia. We evaluated the performance of the instrument using MicroScan Rapid fluorescent panels to determine the identity and antibiotic susceptibilities of 100 Gram negative and 100 Gram positive organisms representing both common clinical isolates and selected organisms of interest. MicroScan results were compared with those obtained by conventional biochemical identification, and antibiotic susceptibility testing using agar dilution following the National Committee on Clinical Laboratory Standards guidelines. MicroScan and reference identifications were in agreement for 93% of Gram negative organisms. MicroScan results were available within 2 hrs. Additional tests were required to confirm the identity of 9 isolates but on only 2 occasions would a definitive identification been delayed beyond 24 hrs. Very major or major discrepancies were seen in 2% and minor discrepancies in 8% of Gram negative susceptibility tests. Susceptibility results were available within 7 hrs but could not be obtained for 13 slow growing organisms. With Gram positive organisms MicroScan agreed with the reference identification of 87% of isolates cultured on horse and 90% of those cultured on sheep blood agar. Discrepancies that occurred in the identification of some streptococci made us question the suitability of MicroScan as the sole means of identifying these organisms. All identifications were available within 24 hrs and the requirement for additional tests was minimal. Susceptibility results closely matched those obtained by agar dilution with < 1% major and 7% and 9% minor discrepancies occurring with sheep and horse blood respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
MicroScan WalkAway是一种自动化细菌鉴定和药敏测试系统，最近才在澳大拉西亚地区上市。我们使用MicroScan快速荧光板评估了该仪器的性能，以确定100株革兰氏阴性菌和100株革兰氏阳性菌的身份及药敏情况，这些菌株代表常见临床分离株和选定的目标菌株。将MicroScan的结果与通过传统生化鉴定以及按照美国国家临床实验室标准委员会指南采用琼脂稀释法进行的药敏测试结果进行比较。对于93%的革兰氏阴性菌，MicroScan与参考鉴定结果一致。MicroScan的结果在2小时内可得。需要额外测试来确认9株菌株的身份，但只有2次最终鉴定延迟超过24小时。在革兰氏阴性菌药敏测试中，极重大或重大差异为2%，微小差异为8%。药敏结果在7小时内可得，但13株生长缓慢的菌株无法得出结果。对于革兰氏阳性菌，MicroScan与在马血琼脂上培养的87%的分离株以及在羊血琼脂上培养的90%的分离株的参考鉴定结果一致。某些链球菌鉴定中出现的差异使我们质疑MicroScan作为鉴定这些菌株的唯一方法的适用性。所有鉴定结果在24小时内可得，且额外测试的需求极少。药敏结果与琼脂稀释法获得的结果密切匹配，在羊血和马血中分别出现<1%的重大差异和7%及9%的微小差异。（摘要截选至250词）
