Levinson W, Mikelens P, Jackson J
Adv Exp Med Biol. 1977;91:161-78. doi: 10.1007/978-1-4684-0796-9_12.
Several, structurally different, copper-binding ligands can inhibit the RNA-dependent DNA polymerase of Rous sarcoma virus (RSV) and can inactivate the ability of the virus to malignantly transform chick embryo cells. These ligands include the anti-microbial agents, thiosemicarbazones, 8-hydroxyquinolines, isonicotinic acid hydrazide, and others. Many of these compounds bind to DNA and RNA in the presence of copper, which may play a role in their anti-viral activity. However, not all agents active against RSV bind to nucleic acids and not all ligands that bind to nucleic acids are active against RSV. Some copper-binding ligands are neither active against RSV, nor bind nucleic acids. It appears that there is no simple relationship between the anti-viral activity of copper-binding ligands and their nucleic acid-binding ability. The biological importance of thiosemicarbazone-copper complex binding to nucleic acids is supported by the observation that treatment of intact RSV virions with the complex causes the genome 70S RNA to sediment abnormally in velocity sucrose gradient analysis.
几种结构不同的铜结合配体可以抑制劳氏肉瘤病毒（RSV）的RNA依赖性DNA聚合酶，并能使该病毒恶性转化鸡胚细胞的能力失活。这些配体包括抗菌剂、硫代卡巴腙、8-羟基喹啉、异烟肼等。其中许多化合物在有铜存在的情况下会与DNA和RNA结合，这可能在它们的抗病毒活性中发挥作用。然而，并非所有对RSV有活性的药物都能与核酸结合，也并非所有与核酸结合的配体都对RSV有活性。一些铜结合配体既对RSV无活性，也不与核酸结合。看来铜结合配体的抗病毒活性与其核酸结合能力之间没有简单的关系。硫代卡巴腙-铜复合物与核酸结合的生物学重要性得到了以下观察结果的支持：用该复合物处理完整的RSV病毒粒子会导致基因组70S RNA在速度蔗糖梯度分析中出现异常沉降。
