Bigi F, Romano M I, Alito A, Cataldi A
Instituto de Biología Molecular, CICV/INTA, Moron, Argentina.
Res Microbiol. 1995 May;146(4):341-8. doi: 10.1016/0923-2508(96)81057-6.
A repetitive DNA from a wild-type Mycobacterium bovis isolate was cloned and characterized. The repeated segment was present in M. bovis and M. tuberculosis but was absent from the six other mycobacteria tested. Sequence analysis demonstrated that this repetitive element belonged to the polymorphic GC-rich repeat sequence type, a family of interspersed repeated DNA. This fragment, when used as a probe in restriction fragment length polymorphism analyses, was able to detect polymorphism in M. bovis genotypes that went undetected when the established IS6110 was used as a probe. This repetitive element should be useful in epidemiological studies of bovine tuberculosis.
从一株野生型牛分枝杆菌分离株中克隆并鉴定了一种重复DNA。该重复片段存在于牛分枝杆菌和结核分枝杆菌中，但在所测试的其他六种分枝杆菌中不存在。序列分析表明，这种重复元件属于富含GC的多态性重复序列类型，是一类散布的重复DNA家族。当该片段用作限制性片段长度多态性分析的探针时，能够检测到牛分枝杆菌基因型中的多态性，而使用已确立的IS6110作为探针时则无法检测到这些多态性。这种重复元件在牛结核病的流行病学研究中应该是有用的。
