牛分枝杆菌中插入序列IS1081的鉴定。
Identification of an insertion sequence, IS1081, in Mycobacterium bovis.
作者信息
Collins D M, Stephens D M
机构信息
Central Animal Health Laboratory, Wallaceville Animal Research Centre, Upper Hutt, New Zealand.
出版信息
FEMS Microbiol Lett. 1991 Sep 15;67(1):11-5. doi: 10.1016/0378-1097(91)90435-d.
An insertion sequence, IS1081, in the genome of Mycobacterium bovis has been identified and sequenced. It is 1324 bp long with 15 bp inverted repeat ends and contains a large ORF. There are six copies of IS1081 in the genome of M. bovis and the element is also present in Mycobacterium tuberculosis. IS1081 is not closely related to other DNA elements described in actinomycetes but its putative transposase bears some resemblance to that of IS256 from Staphylococcus aureus. IS1081 may be useful for genetic manipulations and for developing a diagnostic test for bovine tuberculosis based on the polymerase chain reaction.
已在牛分枝杆菌基因组中鉴定并测序了一个插入序列IS1081。它长1324 bp，末端有15 bp的反向重复序列，并且包含一个大的开放阅读框。在牛分枝杆菌基因组中有6个IS1081拷贝，该元件也存在于结核分枝杆菌中。IS1081与放线菌中描述的其他DNA元件没有密切关系，但其推定的转座酶与金黄色葡萄球菌的IS256的转座酶有一些相似之处。IS1081可能有助于基因操作以及基于聚合酶链反应开发牛结核病诊断测试。
Zotero 插件