Bueno C, Bonfá E D, Radu A S, Cossermelli W
Disciplina de Reumatologia da Faculdade de Medicina, Universidade de São Paulo.
Rev Hosp Clin Fac Med Sao Paulo. 1995 Mar-Apr;50(2):101-6.
The detection of antineutrophil cytoplasmic antibodies is a very important tool for the diagnosis of systemic vasculitis. The specificity and sensitivity of these antibodies depends on the assay utilized for their detection. Therefore we have compared the immunofluorescence test (IF) with the ELISA using two different antigens: total neutrophil extract and isolated primary granules. Two patterns of fluorescence were detected by IF: the classic pattern was highly specific for Wegener's granulomatosis. In contrast the perinuclear staining correlated with renal vasculitis but was also observed in other diseases. However the IF test was unable to differentiate low-positive from atypical patterns. Such distinction could be achieved by ELISA. The use of ELISA with isolated primary granules is a good alternative for if since it has a good specificity, sensitivity and reproducibility, moreover it is a quantitative method.
抗中性粒细胞胞浆抗体的检测是诊断系统性血管炎的一项非常重要的工具。这些抗体的特异性和敏感性取决于用于检测它们的检测方法。因此，我们使用两种不同的抗原：全中性粒细胞提取物和分离的初级颗粒，将免疫荧光试验（IF）与酶联免疫吸附测定（ELISA）进行了比较。通过IF检测到两种荧光模式：经典模式对韦格纳肉芽肿具有高度特异性。相比之下，核周染色与肾血管炎相关，但在其他疾病中也可观察到。然而，IF试验无法区分低阳性和非典型模式。ELISA可以实现这种区分。使用分离的初级颗粒进行ELISA是IF的一个很好的替代方法，因为它具有良好的特异性、敏感性和可重复性，此外它还是一种定量方法。
