Truc P, Bailey J W, Doua F, Laveissière C, Godfrey D G
Department of Veterinary Medicine, University of Bristol, Langford.
Trans R Soc Trop Med Hyg. 1994 Jul-Aug;88(4):419-21. doi: 10.1016/0035-9203(94)90410-3.
The card agglutination test for trypanosomiasis (CATT) was used to examine 8974 inhabitants in 14 village areas south-west of Daloa, Côte d'Ivoire; 114 (1.3%) were CATTT or +/-, and were further examined by one or more of 6 methods for the direct detection of trypanosomes: lymphatic gland puncture, stained thick blood film (TBF), haematocrit centrifugation technique (HCT), mini-anion exchange column (MAEC), quantitative buffy coat method (QBC), and kit for in vitro isolation of trypanosomes (KIVI). Trypanosomes were seen by at least one method in 16 (14.0%) of the CATT+ group. Blood from 356 of the 8860 CATT- group was inoculated into KIVI; trypanosomes grew from the blood of 1 person. Eleven of the 17 patients with detectable trypanosomes were screened by all 6 methods: 6 were HCT+; 7 were gland+; 10 were MAEC+; 10 were KIVI+; 11 were both TBF+ and QBC+. One CATT+ patient was KIVI+ but otherwise negative, although TBF was not done. The overall prevalence of trypanosomes was 0.2% rising to 0.8% in one village area. The results support previous evidence that a reappraisal of procedures is required in the customary system of surveillance for gambian sleeping sickness.
采用锥虫病卡片凝集试验（CATT）对科特迪瓦大洛阿西南部14个村庄地区的8974名居民进行检测；114人（1.3%）CATT检测结果为TT或±，并通过6种直接检测锥虫的方法中的一种或多种进行进一步检测：淋巴结穿刺、染色厚血膜（TBF）、血细胞比容离心技术（HCT）、微型阴离子交换柱（MAEC）、定量血沉棕黄层法（QBC）和锥虫体外分离试剂盒（KIVI）。在CATT阳性组中，至少有一种方法检测到锥虫的有16人（14.0%）。在8860名CATT阴性组中，356人的血液接种到KIVI中；1人的血液中培养出锥虫。17名可检测到锥虫的患者中有11人接受了所有6种方法的筛查：6人HCT阳性；7人淋巴结穿刺阳性；10人MAEC阳性；10人KIVI阳性；11人TBF和QBC均阳性。1名CATT阳性患者KIVI阳性，但其他检测均为阴性，不过未进行TBF检测。锥虫的总体患病率为0.2%，在一个村庄地区升至0.8%。结果支持了先前的证据，即对冈比亚昏睡病常规监测系统的程序需要重新评估。
