McNamara J J, Bailey J W, Smith D H, Wakhooli S, Godfrey D G
MRC Trypanosomiasis Research Group, University of Bristol, Langford, UK.
Trans R Soc Trop Med Hyg. 1995 Jul-Aug;89(4):388-9. doi: 10.1016/0035-9203(95)90021-7.
867 individuals from 3 sites near the town of Adjumani in the East Moyo region of north-west Uganda were investigated clinically and serologically for evidence of current trypanosome infections. Blood samples were taken from 94 persons with a positive card agglutination test for trypanosomiasis (CATT) and clinical suspects and inoculated into the kit for in vitro isolation of Trypanosoma brucei gambiense (KIVI). Amongst this group, 30 parasitaemic individuals were identified by microhaematocrit centrifugation and the quantitative buffy coat technique (QBC). Only 80% of these isolates, and one isolate from an aparasitaemic individual, grew in culture. The success or failure of cultures from parasitaemic patients was unrelated to the size of the trypanosome inoculum. The implications of these results and possible reasons for the failure of KIVI are discussed.
对乌干达西北部东莫约地区阿朱马尼镇附近3个地点的867人进行了临床和血清学调查，以寻找当前锥虫感染的证据。从94名锥虫病卡片凝集试验（CATT）呈阳性的人和临床疑似患者采集血样，并接种到用于体外分离布氏冈比亚锥虫的试剂盒（KIVI）中。在这组人群中，通过微量血细胞比容离心和定量血沉棕黄层技术（QBC）鉴定出30名寄生虫血症患者。这些分离株中只有80%以及1名非寄生虫血症患者的1个分离株在培养中生长。寄生虫血症患者培养的成功或失败与接种锥虫的量无关。讨论了这些结果的意义以及KIVI失败的可能原因。
