Rogers S E, Edmondson D, Goodrick M J, Standen G R, Franck V, Reppucci A, Pamphilon D H
Blood Transfusion Centre, Bristol, UK.
Transfusion. 1995 Sep;35(9):727-33. doi: 10.1046/j.1537-2995.1995.35996029155.x.
Prestorage white cell (WBC) reduction in blood components may decrease the incidence of adverse reactions and improve component quality. A bottom-and-top system with an integral third-generation WBC-reduction filter has been studied.
Whole blood was collected from 30 healthy donors: from 20 by using a blood container system with an integral filter and from 10 controls by using a standard blood container system. Ten test units were buffy coat-depleted, stored for 72 hours at 4 degrees C, and then filtered, while an additional 10 test units were buffy coat-depleted and filtered at room temperature within 8 hours of collection. All units were stored at 4 degrees C for 42 days and sampled weekly.
The mean WBC content of the 72-hour, 4 degrees C units was 0.33 x 10(6), that of the room-temperature units was 2.6 x 10(6), and that of the buffy coat-depleted controls was 460 x 10(6) (p < 0.0005). No significant differences were found among lactate, glucose, sodium, potassium, and plasma hemoglobin levels in the three groups. ATP and 2,3 DPG levels were significantly better preserved in control units than in 72-hour, 4 degrees C units (p = 0.016 and p = 0.032, respectively), but not better than in the room-temperature units. Significant differences were observed between pH values in filtered units and both groups of test units (p = 0.016). In biologic terms however, these differences were small. Red cells from an additional eight healthy volunteer donors were processed by an 8-hour room-temperature method and stored for 35 days. Studies in vivo 24-hour recovery of autologous red cells were performed by transfusing a radiolabeled (51Cr plus 131I-albumin) aliquot after 35 days' storage. Good recovery (mean > 80%) was found by both the single- and double-isotope-label methods. Recovery was significantly greater when calculated by the single-isotope method (p = 0.02).
The combination of buffy coat removal and filtration in the blood container system with an integral filter achieved effective WBC reduction (> or = 3 log10 reduction from whole blood) without biologically significant detriment to in vitro or in vivo storage values.
血液成分预储存时去除白细胞（WBC）可降低不良反应的发生率并提高成分质量。已对一种带有一体式第三代白细胞去除过滤器的上下层系统进行了研究。
从30名健康供者采集全血：20名使用带有一体式过滤器的血袋系统采集，10名使用标准血袋系统作为对照。10个测试单位去除白细胞层，在4℃储存72小时，然后过滤，另外10个测试单位去除白细胞层并在采集后8小时内在室温下过滤。所有单位均在4℃储存42天，每周取样。
4℃储存72小时的单位平均白细胞含量为0.33×10⁶，室温单位为2.6×10⁶，去除白细胞层的对照单位为460×10⁶（p<0.0005）。三组的乳酸、葡萄糖、钠、钾和血浆血红蛋白水平无显著差异。对照单位的ATP和2,3 - DPG水平保存明显优于4℃储存72小时的单位（分别为p = 0.016和p = 0.032），但不优于室温单位。过滤单位与两组测试单位的pH值之间存在显著差异（p = 0.016）。然而，从生物学角度来看，这些差异很小。另外8名健康志愿者供者的红细胞采用8小时室温法处理并储存35天。储存35天后，通过输注放射性标记（⁵¹Cr加¹³¹I - 白蛋白）的等分试样进行自体红细胞体内24小时恢复研究。单同位素标记法和双同位素标记法均发现良好的恢复率（平均>80%）。用单同位素法计算时恢复率显著更高（p = 0.02）。
在带有一体式过滤器的血袋系统中去除白细胞层并进行过滤，可有效降低白细胞（从全血中减少≥3 log₁₀），且对体外或体内储存值无生物学上的显著损害。
