National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, University, MS, USA.
Department of Pharmaceutics and Drug Delivery, School of Pharmacy, The University of Mississippi, University, MS, USA.
Planta Med. 2018 Mar;84(4):260-266. doi: 10.1055/s-0043-124873. Epub 2017 Dec 20.
Cannabinoids are a group of terpenophenolic compounds in the medicinal plant (Cannabaceae family). Cannabigerolic acid, Δ-tetrahydrocannabinolic acid A, cannabidiolic acid, Δ-tetrahydrocannabinol, cannabigerol, cannabidiol, cannabichromene, and tetrahydrocannabivarin are major metabolites in the classification of different strains of . Degradation or artifact cannabinoids cannabinol, cannabicyclol, and Δ-tetrahydrocannabinol are formed under the influence of heat and light during processing and storage of the plant sample. An ultrahigh-performance liquid chromatographic method coupled with photodiode array and single quadruple mass spectrometry detectors was developed and validated for quantitative determination of 11 cannabinoids in different samples. Compounds 1: - 11: were baseline separated with an acetonitrile (with 0.05% formic acid) and water (with 0.05% formic acid) gradient at a flow rate of 0.25 mL/min on a Waters Cortec UPLC C18 column (100 mm × 2.1 mm I. D., 1.6 µm). The limits of detection and limits of quantitation of the 11 cannabinoids were below 0.2 and 0.5 µg/mL, respectively. The relative standard deviation for the precision test was below 2.4%. A mixture of acetonitrile and methanol (80 : 20, /) was proven to be the best solvent system for the sample preparation. The recovery of all analytes was in the range of 97 - 105%. A total of 32 samples including hashish, leaves, and flower buds were analyzed.
大麻素是药用植物（大麻科）中的一组萜酚类化合物。大麻二酚酸、Δ-四氢大麻酸 A、大麻二酚酸、Δ-四氢大麻酚、大麻二醇、大麻二酚、大麻色烯、四氢大麻素和四氢大麻素是不同大麻品种分类中的主要代谢物。大麻酚、大麻环醇和 Δ-四氢大麻酚是在加工和储存植物样品过程中受热和光的影响而形成的降解或人工大麻素。建立并验证了一种超高效液相色谱法，结合光电二极管阵列和单四极杆质谱检测器，用于定量测定不同大麻样品中的 11 种大麻素。化合物 1-11：在 0.25 mL/min 的流速下，用乙腈（含 0.05%甲酸）和水（含 0.05%甲酸）梯度洗脱，在 Waters Cortec UPLC C18 柱（100 mm × 2.1 mm I.D.，1.6 µm）上基线分离。11 种大麻素的检测限和定量限均低于 0.2 和 0.5 µg/mL。精密度试验的相对标准偏差低于 2.4%。证明乙腈和甲醇（80 : 20，/）的混合物是样品制备的最佳溶剂系统。所有分析物的回收率均在 97 - 105%范围内。对包括哈希什、叶子和花蕾在内的 32 个样品进行了分析。
