Chang C P, Chang J Y, Wang F Y, Tseng J, Chang J G
Department of Molecular Medicine & Chinese Medicine, Taipei Municipal Jen-Ai Hospital, Taiwan.
Am J Chin Med. 1995;23(2):173-80. doi: 10.1142/S0192415X95000237.
The effect of Evodia rutaecarpa extract on cytokine secretion by human mononuclear cells in vitro was investigated. Evodia rutaecarpa extract of various concentrations in mononuclear cell culture medium showed biphasic effects on the secretion of interleukin 1 beta (IL-1 beta), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-alpha), and granulocyte-macrophage colony stimulating factor (GM-CSF) by the mononuclear cells. Generally speaking, a low to medium level of Evodia rutaecarpa extract, in concentrations ranging from 10% to 30%, showed significant stimulating effects on the secretion of IL-1 beta, IL-6, TNF-alpha and GM-CSF. On the other hand, high level of Evodia rutaecarpa extract, with concentration more than 40%, lost its stimulating effects. Moreover, reaction time affected the stimulating effects of Evodia rutaecarpa extract on cytokine secretion by mononuclear cells. Mononuclear cell culture medium containing Evodia rutaecarpa extract that was allowed to react for 18 or 24 hours showed significantly better stimulating effects than that reacted for 1 or 3 hours.
研究了吴茱萸提取物对人单核细胞体外细胞因子分泌的影响。在单核细胞培养基中，不同浓度的吴茱萸提取物对单核细胞分泌白细胞介素1β（IL-1β）、白细胞介素6（IL-6）、肿瘤坏死因子α（TNF-α）和粒细胞巨噬细胞集落刺激因子（GM-CSF）呈现双相作用。一般来说，低至中等水平的吴茱萸提取物，浓度范围为10%至30%，对IL-1β、IL-6、TNF-α和GM-CSF的分泌具有显著的刺激作用。另一方面，高浓度的吴茱萸提取物，浓度超过40%，则失去其刺激作用。此外，反应时间影响吴茱萸提取物对单核细胞细胞因子分泌的刺激作用。含有吴茱萸提取物的单核细胞培养基反应18或24小时，其刺激作用明显优于反应1或3小时的情况。
