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与Hémoline双相法相比,Vital系统用于需氧血培养检测的临床评估。

Clinical evaluation of the Vital system compared with the Hémoline diphasic method for the detection of aerobic blood cultures.

作者信息

Avril J L, Mathieu D, Saulnier C, Hignard M

机构信息

Laboratoire de bactériologie-virologie, Centre hospitalier régional et universitaire, Pontchaillou, Rennes, France.

出版信息

Ann Biol Clin (Paris). 1995;53(1-2):21-4.

PMID:7574086
Abstract

In a collaborative study at two university hospitals, the recovery of microorganisms and the speed of detection of microbial aerobic growth by the Vital system (bioMérieux) and a diphasic conventional blood culture system were compared. The Vital system monitors each blood culture bottle every 15 minutes to detect inactivation of fluorescent suspended in the broth medium due to microbial growth. A total of 1086 comparisons were made between the two systems, yielding a total of 117 isolates. Microorganisms were recovered more often from the Vital aerobic bottles (p < 0.05). The Vital system detected 43% of the microorganisms within the first 12 hours of incubation whereas in the same time the conventional system detected only 5% of the microorganisms. The results demonstrate that the Vital system is a reliable, continuous monitoring, fully automated system and an attractive alternative to conventional blood culture methods.

摘要

在两家大学医院开展的一项合作研究中,对Vital系统(生物梅里埃公司)和双相传统血培养系统的微生物回收率及微生物需氧生长的检测速度进行了比较。Vital系统每15分钟监测一次每个血培养瓶,以检测由于微生物生长导致的肉汤培养基中悬浮荧光物质的失活情况。两个系统之间共进行了1086次比较,共分离出117株菌株。从Vital需氧瓶中回收微生物的情况更为常见(p < 0.05)。Vital系统在培养的前12小时内检测到了43%的微生物,而在同一时间段内,传统系统仅检测到了5%的微生物。结果表明,Vital系统是一个可靠的、可连续监测的全自动系统,是传统血培养方法的一个有吸引力的替代方案。

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