Katouli M, Lund A, Wallgren P, Kühn I, Söderlind O, Möllby R
Laboratory for Bacteriology, Karolinska Institute, Stockholm, Sweden.
Appl Environ Microbiol. 1995 Feb;61(2):778-83. doi: 10.1128/aem.61.2.778-783.1995.
A highly discriminatory and standardized biochemical fingerprinting method was used to monitor the persistence and colonization of intestinal Escherichia coli isolated from the feces of four sows and their litters (four piglets from each) during the suckling, postweaning, and fattening periods. Altogether, 195 fecal samples were collected and 1,827 E. coli strains were tested (mean number of isolates tested per fecal sample per pig, 9.5). Strains were divided into similarity groups on the basis of their biochemical phenotypes (BPTs). The diversity of E. coli strains in each sample was measured with Simpson's index of diversity, and similarity between E. coli floras of piglets was calculated with a population similarity index. Each fecal sample contained several BPTs of E. coli, some of which dominated that population. The intestinal colonization of piglets consisted of successive waves of different E. coli BPTs, the tenure of which varied from a few days to 2 weeks. Most of these BPTs disappeared in the succeeding samples and were not recovered again from the same piglets. On the other hand, some E. coli strains which colonized piglets early during the suckling period persisted for a long period and were referred to as resident BPTs. Each piglet carried more than one resident BPT (mean of 2.4 BPTs per pig), some of which were also found in other piglets.(ABSTRACT TRUNCATED AT 250 WORDS)
一种具有高度鉴别性和标准化的生化指纹图谱方法被用于监测从4头母猪及其仔猪（每头母猪4头仔猪）粪便中分离出的肠道大肠杆菌在哺乳、断奶后和育肥期的持续性和定植情况。总共收集了195份粪便样本，检测了1827株大肠杆菌（每头猪每份粪便样本检测的分离株平均数为9.5株）。菌株根据其生化表型（BPTs）被分为相似性组。每个样本中大肠杆菌菌株的多样性用辛普森多样性指数来衡量，仔猪大肠杆菌菌群之间的相似性用种群相似性指数来计算。每份粪便样本都含有几种大肠杆菌的BPTs，其中一些在该菌群中占主导地位。仔猪的肠道定植由不同大肠杆菌BPTs的连续浪潮组成，其持续时间从几天到2周不等。这些BPTs中的大多数在后续样本中消失，并且没有从同一仔猪中再次分离到。另一方面，一些在哺乳早期定植于仔猪的大肠杆菌菌株长期存在，被称为常驻BPTs。每头仔猪携带不止一种常驻BPT（每头猪平均2.4种BPTs），其中一些也在其他仔猪中被发现。（摘要截短于250字）
