从污水中分离出的轮状病毒的基因分型。
Genotyping of rotaviruses isolated from sewage.
作者信息
Gajardo R, Bouchriti N, Pintó R M, Bosch A
机构信息
Department of Microbiology, University of Barcelona, Spain.
出版信息
Appl Environ Microbiol. 1995 Sep;61(9):3460-2. doi: 10.1128/aem.61.9.3460-3462.1995.
Abstract
Rotaviruses from environmental samples have been genotyped by a seminested reverse transcription PCR assay with serotype-specific primers derived from variable regions of gene 9, which produce different characteristic segment sizes for serotypes 1 to 4. The method enabled the detection and identification of type 1, 2, and 3 group A rotaviruses in sewage.
摘要
通过半巢式逆转录聚合酶链反应（PCR）检测法，利用源自基因9可变区的血清型特异性引物，对环境样本中的轮状病毒进行基因分型，该引物可针对血清型1至4产生不同特征性片段大小。该方法能够检测和鉴定污水中的1型、2型和3型A组轮状病毒。
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