Paget S A, Anderson K, Phillips P E
Arthritis Rheum. 1978 Mar;21(2):249-55. doi: 10.1002/art.1780210213.
A sensitive complement-dependent chromium release cytotoxicity assay was used to determine whether sera from rheumatoid arthritis (RA) patients contain antibody specific for an antigen on rheumatoid synovial cell cultures. Two hundred eight RA sera-RA synovial culture combinations were studied employing 21 sera and 16 synovial membranes; control combinations were derived from 5 normal sera and 10 degenerative joint disease synovial membranes. Anticomplementary activity of some rheumatoid sera was overcome using an increased complement concentration. The percent cytotoxicity of RA serum-RA culture combinations, both homologous and autologous, was not significantly greater than that of RA serum-control culture combinations. No correlation between duration of disease or duration of cell culture and percent cytotoxicity was found. Thus a unique antigen on cultured rheumatoid synovial cells was not recognized by rheumatoid serum antibody by use of this cytotoxicity assay.
采用一种灵敏的补体依赖的铬释放细胞毒性试验，以确定类风湿关节炎（RA）患者的血清中是否含有针对类风湿滑膜细胞培养物上某种抗原的特异性抗体。使用21份血清和16块滑膜组织，研究了208种RA血清-RA滑膜培养物组合；对照组合来自5份正常血清和10份退行性关节病滑膜组织。通过增加补体浓度克服了一些类风湿血清的抗补体活性。RA血清-RA培养物组合（包括同源和自体组合）的细胞毒性百分比并不显著高于RA血清-对照培养物组合。未发现疾病持续时间或细胞培养持续时间与细胞毒性百分比之间存在相关性。因此，通过这种细胞毒性试验，类风湿血清抗体未识别出培养的类风湿滑膜细胞上的独特抗原。
