Bridge P D, Hopkinson L A, Rutherford M A
International Mycological Institute, Egham, Surrey, UK.
Lett Appl Microbiol. 1995 Sep;21(3):198-201. doi: 10.1111/j.1472-765x.1995.tb01040.x.
A method is described for the production of simple mitochondrial DNA probes from filamentous fungi for the partial characterization of mitochondrial DNA without the need for cloning, gradient centrifugation or PCR amplification. A probe (P449) consisting of a 3.38 kb mitochondrial fragment from an isolate of Fusarium oxysporum special form cubense was used to determine RFLPs in restriction digests of total DNA from 28 isolates of F. oxysporum from a variety of hosts and locations. The probe showed mtDNA polymorphisms within and between different special forms.
本文描述了一种从丝状真菌中制备简单线粒体DNA探针的方法，用于线粒体DNA的部分特征分析，无需克隆、梯度离心或PCR扩增。使用由尖孢镰刀菌古巴专化型分离株的3.38 kb线粒体片段组成的探针（P449）来确定来自不同宿主和地点的28株尖孢镰刀菌总DNA限制性消化产物中的RFLP。该探针在不同专化型内和不同专化型之间均显示出线粒体DNA多态性。
