Grönroos J M, Kuttila K, Perttilä J, Nevalainen T J
Department of Surgery, University of Turku, Finland.
Eur J Clin Chem Clin Biochem. 1995 May;33(5):271-4. doi: 10.1515/cclm.1995.33.5.271.
Phospholipase A2 has been implicated in the pathogenesis of local and distant tissue injury after ischaemia and reperfusion. A common operation inducing ischaemia and reperfusion is aortobifemoral reconstruction, during which the aorta is clamped and the blood supply via the inferior mesenteric artery and iliac arteries is interrupted. The purpose of the present work was to study the catalytic activity concentration of phospholipase A2 and the mass concentrations of group I and group II phospholipases A2 in the sera of patients undergoing aortobifemoral reconstruction. Both the catalytic activity concentration of phospholipase A2 and the mass concentrations of group I and group II phospholipases A2 increased in serum samples after the operation. The catalytic activity concentration of phospholipase A2 correlated well with group II phospholipase A2 mass values (r = 0.81, p < 0.001), whereas no correlation was found between the catalytic activity concentration of phospholipase A2 and group I phospholipase A2 mass values (r = 0.12, p = 0.54). The results emphasize the role of group II phospholipase A2 in tissue injury after ischaemia and reperfusion.
磷脂酶A2与缺血再灌注后局部和远处组织损伤的发病机制有关。一种常见的诱导缺血再灌注的手术是腹主动脉双股动脉重建术，在此过程中，主动脉被夹住，通过肠系膜下动脉和髂动脉的血液供应被中断。本研究的目的是研究接受腹主动脉双股动脉重建术患者血清中磷脂酶A2的催化活性浓度以及I组和II组磷脂酶A2的质量浓度。术后血清样本中磷脂酶A2的催化活性浓度以及I组和II组磷脂酶A2的质量浓度均升高。磷脂酶A2的催化活性浓度与II组磷脂酶A2质量值密切相关（r = 0.81，p < 0.001），而磷脂酶A2的催化活性浓度与I组磷脂酶A2质量值之间未发现相关性（r = 0.12，p = 0.54）。结果强调了II组磷脂酶A2在缺血再灌注后组织损伤中的作用。
