Laine V J, Grönroos J M, Nevalainen T J
Department of Pathology, University of Turku, Finland.
Eur J Clin Chem Clin Biochem. 1996 May;34(5):419-22.
Phospholipase A2 values increase in serum in various inflammatory states, infections, and postoperatively in surgical patients. Several organs, including the liver and spleen have been suggested as sources of circulating phospholipase A2. The purpose of the present work was to examine the possible role of the spleen as a source of elevated serum concentrations of phospholipase A2 after surgery. Pre- and postoperative serum samples of patients undergoing splenectomy were studied for group I phospholipase A2, group II phospholipase A2, and C-reactive protein mass concentrations and catalytic activity concentration of phospholipase A2. The catalytic activity concentration of phospholipase A2 and the mass concentrations of group II phospholipase A2 and C-reactive protein increased postoperatively (8.08 +/- 1.40 U/l vs. 3.96 +/- 0.89 U/l (mean +/- SEM) for phospholipase A2 catalytic concentration (p < 0.03), and 154.8 +/- 32.1 micrograms/l vs. 47.5 +/- 14.7 micrograms/l (mean +/- SEM) for group II phospholipase A2 mass concentration (p < 0.02, n = 7). The mass concentration of group I phospholipase A2 remained unchanged. The catalytic concentration of phospholipase A2 correlated well with the mass concentration of group II phospholipase A2 (p < 0.001, r = 0.846, n = 43). The concentration of C-reactive protein correlated well with the mass concentration of group II phospholipase A2 (p < 0.001, r = 0.566, n = 43) in serum. The results indicate that group II phospholipase A2 is released into the circulation after splenectomy, and the spleen seems not to be the source of circulating group II phospholipase A2.
在各种炎症状态、感染以及外科手术患者术后，血清中的磷脂酶A2值会升高。包括肝脏和脾脏在内的多个器官被认为是循环中磷脂酶A2的来源。本研究的目的是探讨脾脏作为术后血清磷脂酶A2浓度升高来源的可能作用。对接受脾切除术患者术前和术后的血清样本进行了研究，检测了I组磷脂酶A2、II组磷脂酶A2、C反应蛋白的质量浓度以及磷脂酶A2的催化活性浓度。术后磷脂酶A2的催化活性浓度以及II组磷脂酶A2和C反应蛋白的质量浓度升高（磷脂酶A2催化浓度：8.08±1.40 U/l，术前为3.96±0.89 U/l（均值±标准误），p<0.03；II组磷脂酶A2质量浓度：154.8±32.1 μg/l，术前为47.5±14.7 μg/l（均值±标准误），p<0.02，n = 7）。I组磷脂酶A2的质量浓度保持不变。磷脂酶A2的催化浓度与II组磷脂酶A2的质量浓度密切相关（p<0.001，r = 0.846，n = 43）。血清中C反应蛋白的浓度与II组磷脂酶A2的质量浓度密切相关（p<0.001，r = 0.566，n = 43）。结果表明，脾切除术后II组磷脂酶A2释放入循环，脾脏似乎不是循环中II组磷脂酶A2的来源。
