Steinmetz J, Tarallo P, Fournier B, Caces E, Siest G
Centre de Médecine Préventive, URA CNRS 597, Vandoeuvre-Les-Nancy, France.
Eur J Clin Chem Clin Biochem. 1995 Jun;33(6):337-42. doi: 10.1515/cclm.1995.33.6.337.
An important collaborative study organized by the IFCC enabled the selection of international reference materials and the standardization of commercially available methods by the use of common calibrators. In this paper, we report the reference limits of apolipoprotein A-I and apolipoprotein B in a selected healthy French population. The apolipoprotein measurements were performed on BNA Behring using reagents and protocols supplied by the manufacturer: the standard sera were calibrated using the IFCC-WHO reference preparations (SP1 and SP3). In addition, the apolipoprotein B protocol was modified by the addition of a supplementary reagent to reduce the interference by lipaemic samples on immunonephelometric measurement. In a sample of 115 random serum samples, there was a decrease in mean concentration between non-standardized and standardized methods: -4.8% for apolipoprotein A-I and -4.7% for apolipoprotein B. The reference limits for apolipoprotein A-I are unaffected by gender between 4 and 14 years, thereafter vary with age and gender until 40 years and with gender alone between 40 and 60 years. For apolipoprotein B, the variation with gender is only significant between 30 and 49 years.
由国际临床化学和检验医学联合会（IFCC）组织的一项重要合作研究，通过使用通用校准物，实现了国际参考物质的选择以及市售方法的标准化。在本文中，我们报告了在选定的健康法国人群中载脂蛋白A-I和载脂蛋白B的参考限值。载脂蛋白的测量在BNA Behring上进行，使用制造商提供的试剂和方案：标准血清使用IFCC-世界卫生组织（WHO）参考制剂（SP1和SP3）进行校准。此外，通过添加一种补充试剂对载脂蛋白B的检测方案进行了改进，以减少脂血样本对免疫比浊法测量的干扰。在115份随机血清样本中，非标准化方法和标准化方法之间的平均浓度有所下降：载脂蛋白A-I下降了4.8%，载脂蛋白B下降了4.7%。载脂蛋白A-I的参考限值在4至14岁之间不受性别影响，此后随年龄和性别变化直至40岁，在40至60岁之间仅随性别变化。对于载脂蛋白B，仅在30至49岁之间性别差异显著。
