Boerma G J, de Bruijn A M, van Teunenbroek A
Department of Clinical Chemistry, Sophia Childrens University Hospital, Rotterdam, The Netherlands.
Eur J Clin Chem Clin Biochem. 1994 Dec;32(12):923-7. doi: 10.1515/cclm.1994.32.12.923.
In a study with young children receiving growth hormone treatment, it was necessary to re-establish reference values for various blood components, e.g. apolipoproteins A-I and B. We find that considerable method-to-method variation still exists. We used the DuPont Dimension analyzer (D) and the Beckman Array analyzer (B) systems and a third procedure with Orion reagents and a Kone Analyzer (K). We investigated if assay results may be pooled or exchanged within our study. In 59 serum samples we measured apolipoprotein A-I and apolipoprotein B (n = 58) and calculated the orthogonal regression equations y = a(Sa) x + b(Sb). For apolipoprotein A-I the results are: (I) B = 1.165 (0.065) D - 0.193 (0.077), Syx = 0.055; with r = 0.922 and (II) K = 0.831 (0.056) D - 0.190 (0.066), Syx = 0.055; with r = 0.898. For apolipoprotein B the equations are: (III) B = 1.586 (0.137) D - 0.246 (0.100), Syx = 0.061; with r = 0.840 and (IV) K = 0.869 (0.065) D + 0.251 (0.048), Syx = 0.044; with r = 0.875. According to Passing & Bablok, the slopes and intercept values are 1.093 and -0.126; 0.848 and 0.167; 1.500 and -0.185; 0.880 and 0.249. The overall impression is the same for both regression methods: comparability had not yet been achieved by early 1993, particularly not for apolipoprotein B, and reference values differ significantly depending on the selected methodology.
在一项针对接受生长激素治疗的幼儿的研究中，有必要重新确定各种血液成分（如载脂蛋白A-I和B）的参考值。我们发现方法间仍存在相当大的差异。我们使用了杜邦Dimension分析仪（D）和贝克曼Array分析仪（B）系统，以及采用奥立龙试剂和康尔全自动生化分析仪（K）的第三种方法。我们研究了在我们的研究中测定结果是否可以合并或互换。在59份血清样本中，我们测定了载脂蛋白A-I和载脂蛋白B（n = 58），并计算了正交回归方程y = a(Sa) x + b(Sb)。对于载脂蛋白A-I，结果如下：（I）B = 1.165 (0.065) D - 0.193 (0.077)，Syx = 0.055；r = 0.922；以及（II）K = 0.831 (0.056) D - 0.190 (0.066)，Syx = 0.055；r = 0.898。对于载脂蛋白B，方程如下：（III）B = 1.586 (0.137) D - 0.246 (0.100)，Syx = 0.061；r = 0.840；以及（IV）K = 0.869 (0.065) D + 0.251 (0.048)，Syx = 0.044；r = 0.875。根据帕辛和巴布洛赫方法，斜率和截距值分别为1.093和 -0.126；0.848和0.167；1.500和 -0.185；0.880和0.249。两种回归方法的总体印象相同：到1993年初尚未实现可比性，特别是载脂蛋白B，并且参考值根据所选方法的不同而有显著差异。
