犬内毒素休克期间纤溶酶原水平、纤溶酶活性及抗纤溶酶活性的变化

Changes in plasminogen levels, plasmin activity and activity of antiplasmins during endotoxin shock in dogs.

作者信息

Aasen A O, Ohlsson K, Larsbraaten M, Amundsen E

出版信息

Eur Surg Res. 1978;10(1):63-72. doi: 10.1159/000127993.

Abstract

Antiplasmin activity was studied during various stages of lethal canine endotoxin shock by means of assays utilizing a new chromogenic tripeptide derivative (S-2251 AB Kabi Peptide Research Division, Mölndal, Sweden). By applying a preincubation time of 30 sec and 5 min, respectively, 'immediate' and 'time-dependent' antiplasmin activities were determined. During shock gradually decreasing values of 'immediate' antiplasmin activity was observed. 'Time-dependent' antiplasmin activity also revealed a decreasing pattern, these changes, however, were less pronounced when compared with 'immediate' antiplasmin activity. The changes of plasma antiplasmin activities observed were accompanied by decreasing values of plasminogen and evidence of plasmin activity. alpha-Antitrypsin and alpha2-Macroglobulin quantitated with electroimmunoassay technique also revealed decreasing patterns during shock.

摘要

利用一种新的生色三肽衍生物（S - 2251，瑞典莫恩达尔AB卡比肽研究部），通过检测法研究了致死性犬内毒素休克不同阶段的抗纤溶酶活性。分别采用30秒和5分钟的预孵育时间，测定“即时”和“时间依赖性”抗纤溶酶活性。在休克期间，观察到“即时”抗纤溶酶活性值逐渐降低。“时间依赖性”抗纤溶酶活性也呈现下降趋势，然而，与“即时”抗纤溶酶活性相比，这些变化不太明显。观察到的血浆抗纤溶酶活性变化伴随着纤溶酶原值的降低和纤溶酶活性的证据。用电免疫测定技术定量的α1 - 抗胰蛋白酶和α2 - 巨球蛋白在休克期间也呈现下降趋势。