Lamour A, Le Corre R, Pennec Y L, Cartron J, Youinou P
Laboratory of Immunology, Brest University Medical School, France.
Blood. 1995 Nov 1;86(9):3553-9.
Our aims were to determine the prevalence of neutrophil antibodies in patients with primary Sjögren's syndrome (pSS), identify their target antigen(s), and evaluate their functional significance. Neutrophil antibodies were detected using an indirect immunofluorescence (IIIF) test and an enzyme-linked immunosorbent assay (ELISA), using recombinant human Fc-gamma receptor (Fc gamma RIIIb) as a capture agent. Luminol-dependent chemiluminescence was then measured by an established technique. Antibodies to neutrophils were detected in 30 of 66 patients (45%) and categorized on the basis of positivity for the two assays: IIF+/ELISA+ (group A: five patients), IIF+/ELISA- (group B: five patients), and IFF-/ELISA+ (group C: 20 patients). All positive sera contained antibodies directed to the neutrophil specific Fc gamma RIIIb, and none of them bound to NAnull neutrophils. The titer of neutrophil-reactive antibodies (groups A and B) showed no correlation with the neutrophil count, but these autoantibodies did reduce the cell ability to generate a respiratory burst. Thus, neutrophil antibodies are common in patients with pSS. Their main target appears to be Fc gamma RIII, and this may partly account for the dysfunction in Fc gamma R-mediated clearance by the reticuloendothelial system reported in these patients.
我们的目的是确定原发性干燥综合征（pSS）患者中中性粒细胞抗体的患病率，识别其靶抗原，并评估其功能意义。使用间接免疫荧光（IIIF）试验和酶联免疫吸附测定（ELISA）检测中性粒细胞抗体，以重组人Fc-γ受体（FcγRIIIb）作为捕获剂。然后通过一种既定技术测量鲁米诺依赖性化学发光。在66例患者中的30例（45%）检测到抗中性粒细胞抗体，并根据两种检测方法的阳性结果进行分类：IIF+/ELISA+（A组：5例患者）、IIF+/ELISA-（B组：5例患者）和IFF-/ELISA+（C组：20例患者）。所有阳性血清均含有针对中性粒细胞特异性FcγRIIIb的抗体，且均未与NAnull中性粒细胞结合。中性粒细胞反应性抗体（A组和B组）的滴度与中性粒细胞计数无关，但这些自身抗体确实降低了细胞产生呼吸爆发的能力。因此，中性粒细胞抗体在pSS患者中很常见。其主要靶标似乎是FcγRIII，这可能部分解释了这些患者中报道的网状内皮系统FcγR介导的清除功能障碍。
