Sali A
The Rockefeller University, New York, USA.
Curr Opin Biotechnol. 1995 Aug;6(4):437-51. doi: 10.1016/0958-1669(95)80074-3.
A protein sequence with at lease 40% identity to a known structure can now be modelled automatically, with an accuracy approaching that o fa low-resolution X-ray structure or a medium-resolution nuclear magnetic resonance structure. In general, these models have goods stereochemistry and an overall structural accuracy that is as high as the similarity between the template and the actual structure being predicted. As a result, the number of sequences that can be modelled is an order of magnitude larger then the number of experimentally determined protein structures. In addition, evaluation techniques are available that can estimated errors in different regions of the model. Thus, the number of applications where homology modelling is proving useful is growing rapidly.
现在，与已知结构具有至少40%同一性的蛋白质序列可以自动建模，其准确性接近低分辨率X射线结构或中等分辨率核磁共振结构。一般来说，这些模型具有良好的立体化学性质和较高的整体结构准确性，与模板和预测的实际结构之间的相似度一样高。因此，可以建模的序列数量比通过实验确定的蛋白质结构数量大一个数量级。此外，还有评估技术可用于估计模型不同区域的误差。因此，同源建模证明有用的应用数量正在迅速增加。
