Dabrowski J, Schönberg A, Wegner Z, Stańczak J, Kruminis-Lozowska W
Institute of Maritime and Tropical Medicine, Gdynia, Poland.
Bull Inst Marit Trop Med Gdynia. 1993;44-45(1-4):61-4.
Among ticks Ixodes ricinus collected in April 1994 in the forest near Fraknowo (Olsztyn province, N. C. Poland), 17 ticks were evaluated for Borrelia infection by cultivating in the BSK-H medium (Sigma). The ticks were examined in five pools--four of 3 females and one pool of 5 nymphs. Spirochaetes were cultured successfully only from one pool of females and identified as Borrelia burgdorferi with monoclonal antibodies: H 9724, H 5332, H 3TS and 11 G 1 by indirect immunofluorescence assay (IFA). This is the first reported isolate of B. burgdorferi from Ixodes ricinus in Poland.
1994年4月在弗拉科诺沃(波兰北部奥尔什丁省)附近森林中采集的蓖麻硬蜱中,选取17只蜱通过在BSK - H培养基(西格玛)中培养来评估伯氏疏螺旋体感染情况。这些蜱被分成五个组——四个组每组3只雌性蜱,一个组5只若虫。仅从一组雌性蜱中成功培养出螺旋体,并通过间接免疫荧光试验(IFA)用单克隆抗体H 9724、H 5332、H 3TS和11 G 1鉴定为伯氏疏螺旋体。这是波兰首次报道从蓖麻硬蜱中分离出伯氏疏螺旋体。