Vaidya R, Atanasov P, Wilkins E
Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque 87131, USA.
Med Eng Phys. 1995 Sep;17(6):416-24. doi: 10.1016/1350-4533(94)00006-u.
The negatively charged perfluorinated ionomer Nafion was used as a coating on hydrogen peroxide detecting platinum electrode as well as on a polycarbonate diffusion membrane in the construction of a glucose amperometric enzyme electrode. The current response of these electrodes to hydrogen peroxide, ascorbic acid, acetaminophen, uric acid and glucose was studied and the coating procedure was optimized. It was confirmed that the Nafion coating prevents interference by anionic substances such as ascorbic acid and uric acid, and decreases acetaminophen interference. In this regard it was shown that coating the glucose diffusion membrane (polycarbonate) was more effective than coating the platinum wire itself, because of the prevention of the homogeneous redox reaction of the interference species with hydrogen peroxide, as well as the additional diffusional resistance to the glucose flux. The glucose levels in serum samples were estimated and the stability of the enzyme electrodes during continuous operation in serum was studied. An enzyme electrode with constant sensitivity of ca. 1 microA/mM and a linear range of up to 15 mM, unaffected by contact with serum, is reported.
带负电荷的全氟离子聚合物Nafion被用作过氧化氢检测铂电极以及聚碳酸酯扩散膜的涂层，用于构建葡萄糖安培酶电极。研究了这些电极对过氧化氢、抗坏血酸、对乙酰氨基酚、尿酸和葡萄糖的电流响应，并对涂层工艺进行了优化。证实Nafion涂层可防止抗坏血酸和尿酸等阴离子物质的干扰，并减少对乙酰氨基酚的干扰。在这方面，研究表明，涂覆葡萄糖扩散膜（聚碳酸酯）比涂覆铂丝本身更有效，这是因为它能防止干扰物质与过氧化氢发生均相氧化还原反应，以及对葡萄糖通量产生额外的扩散阻力。评估了血清样品中的葡萄糖水平，并研究了酶电极在血清中连续运行期间的稳定性。报道了一种酶电极，其灵敏度恒定约为1微安/毫摩尔，线性范围高达15毫摩尔，不受与血清接触的影响。
